Departamento de Bioquímica y Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Salvador Díaz Mirón s/n, Casco de Santo Tomás, Ciudad de México 11340, Mexico.
Instituto Politécnico Nacional, ESFM, Av. Instituto Politécnico Nacional s/n, Edif. 9 U.P. Zacatenco, Col. San Pedro Zacatenco, Ciudad de México 07738, Mexico.
Oxid Med Cell Longev. 2020 Oct 12;2020:3970860. doi: 10.1155/2020/3970860. eCollection 2020.
N-aryl maleimides can undergo a 1,4-Michael-type addition reaction with reduced glutathione (GSH), leading to a decreased concentration of GSH and an increased concentration of free radicals (FRs) in cells. GSH is a critical scavenging molecule responsible for protecting cells from oxidation and for maintaining redox homeostasis. N-aryl maleimides disturb redox homeostasis in cells because they scavenge thiol-containing molecules, especially GSH. This study aimed at measuring the concentrations of GSH and FRs by electronic paramagnetic resonance (EPR), in the brain and liver tissue of male Wistar rats () at different ages and after treatment with 3,5-dimaleimylbenzoic acid (3,5-DMB). Our results showed a relationship between age and the concentrations of GSH and FRs in cells. In young rats, the concentration of GSH was higher than in old rats, while the concentration of FRs was higher in adult rats than in young rats, suggesting an inverse relationship between GSH and FRs. On the other hand, the reaction of 3,5-DMB (an electrophilic maleimide) with cellular GSH increased the FR content. The results of this study contribute to the awareness that the process of aging implies not only a loss of tissue function but also essential changes in the molecular contents of cells, especially the concentrations of FRs and GSH.
N-芳基马来酰亚胺可与还原型谷胱甘肽 (GSH) 发生 1,4-Michael 型加成反应，导致细胞内 GSH 浓度降低和自由基 (FRs) 浓度增加。GSH 是一种关键的清除分子，负责保护细胞免受氧化，并维持氧化还原平衡。N-芳基马来酰亚胺通过清除含巯基的分子，特别是 GSH，扰乱细胞内的氧化还原平衡。本研究旨在通过电子顺磁共振 (EPR) 测量雄性 Wistar 大鼠 () 不同年龄和 3,5-二马来酰基苯甲酸 (3,5-DMB) 处理后的大脑和肝脏组织中 GSH 和 FRs 的浓度。我们的结果显示了年龄与细胞内 GSH 和 FRs 浓度之间的关系。在幼鼠中，GSH 的浓度高于老年鼠，而 FRs 的浓度在成年鼠中高于幼鼠，表明 GSH 和 FRs 之间呈反比关系。另一方面，3,5-DMB（一种亲电马来酰亚胺）与细胞内 GSH 的反应增加了 FR 含量。这项研究的结果有助于认识到衰老过程不仅意味着组织功能的丧失，而且还意味着细胞内分子含量的基本变化，特别是 FRs 和 GSH 的浓度。
