Department of Medicine, Division of Gastroenterology, University of Minnesota, Minneapolis, MN 55414, USA.
BioTechnology Institute, University of Minnesota, St. Paul, MN 55108, USA.
STAR Protoc. 2020 Jul 24;1(2):100071. doi: 10.1016/j.xpro.2020.100071. eCollection 2020 Sep 18.
, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol that can be practiced in any lab setting using a portable anaerobic glove bag. This protocol also optimizes existing cell growth methods and presents a detailed trouble shooting guide. This protocol is a modification of those previously reported by Edwards and McBride (2016) and Shen et al. (2016).
艰难梭菌是一种专性厌氧菌，可导致感染并引起长时间腹泻。该细菌产生休眠孢子，能够耐受有氧环境，从而易于在环境中转移。在这里，我们提供了一个方便的孢子形成和纯化方案，该方案可在任何实验室环境中使用便携式厌氧手套袋进行操作。该方案还优化了现有的细胞生长方法，并提供了详细的故障排除指南。本方案是对 Edwards 和 McBride（2016 年）以及 Shen 等人（2016 年）先前报道的方案的修改。
