Microbiome and Host Health, South Australia Health and Medical Research Institute, Adelaide 5000, Australia.
Microbiome Research Laboratory, College of Medicine and Public Health, Flinders University, Bedford Park 5042, Australia.
STAR Protoc. 2021 Jun 16;2(3):100610. doi: 10.1016/j.xpro.2021.100610. eCollection 2021 Sep 17.
The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).
无菌小鼠的使用对于理解宿主-肠道微生物组关系至关重要。此类模型依赖于供体微生物组的忠实复制，以确定肠道微生物组对宿主病理生理学的因果影响。本方案介绍了供体微生物组的准备和转移，重点是严格的厌氧处理方法和通过灌胃进行多次灌输，以实现最佳的肠道微生物组回收。有关该方案的生成和使用的完整详细信息，请参阅 Choo 和 Rogers（2021 年）。
