Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai 200120, China.
STAR Protoc. 2020 Sep 3;1(2):100101. doi: 10.1016/j.xpro.2020.100101. eCollection 2020 Sep 18.
We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration function . The protocol is also suitable for live-cell imaging of other adherent cells, including fibroblasts and hepatocytes. For complete details on the use and execution of this protocol, please refer to Gong et al. (2014) and Gong et al. (2015).
我们描述了一种使用共聚焦显微镜在活心肌细胞中成像线粒体荧光瞬变增加事件（Mitoflash）的方案。Mitoflash 通过线粒体靶向的环状排列荧光蛋白（mt-cpYFP）检测，可以用于评估线粒体呼吸功能。该方案也适用于其他贴壁细胞的活细胞成像，包括成纤维细胞和肝细胞。有关此方案使用和执行的完整详细信息，请参阅 Gong 等人（2014 年）和 Gong 等人（2015 年）。
