Department of Oncology, Wayne State University School of Medicine, Detroit, MI, USA.
Department of Pathology, Anatomy and Laboratory Medicine, West Virginia University School of Medicine, Morgantown, WV, USA.
Breast Cancer Res Treat. 2021 Feb;185(3):831-839. doi: 10.1007/s10549-020-05983-x. Epub 2020 Oct 28.
Markers of inflammation, including crown-like structures of the breast (CLS-B) and infiltrating lymphocytes (IL), have been identified in breast tissue and associated with increased risk of breast cancer (BrCa), however most of this work has been performed in primarily non-Hispanic white women. Here, we examined whether CLS-B and IL are associated with invasive BrCa in African American (AA) women.
We assessed breast biopsies from three 5-year age-matched groups: BrCa-free AA women (50 Volunteer) from the Komen Normal Tissue Bank (KTB) and AA women with a clinically-indicated biopsy diagnosed with benign breast disease (BBD) from our Detroit cohort who developed BrCa (55 BBD-cancer) or did not develop BrCa (47 BBD only, year of biopsy matched to BBD-cancer). Mean adipocyte diameter and total adipose area were estimated from digital images using the Adiposoft plugin from ImageJ. Associations between CLS-B, IL, and BrCa among KTB and Detroit biopsies were assessed using multivariable multinomial and conditional logistic regression models.
Among all biopsies, Volunteer and BBD only biopsies did not harbor CLS-B or IL at significantly different rates after adjusting for logarithm of adipocyte area, adipocyte diameter, and BMI. Among clinically-indicated BBD biopsies, BBD-cancer biopsies were more likely to exhibit CLS-B (odds ratio (OR) = 3.36, 95% Confidence Interval (CI): 1.33-8.48) or IL (OR = 4.95, 95% CI 1.76-13.9) than BBD only biopsies after adjusting for total adipocyte area, adipocyte diameter, proliferative disease, and BMI.
CLS-B and IL may serve as histological markers of BrCa risk in benign breast biopsies from AA women.
炎症标志物，包括乳腺冠状结构（CLS-B）和浸润淋巴细胞（IL），已在乳腺组织中被鉴定出来，并与乳腺癌（BrCa）风险增加相关，然而，大多数此类工作是在主要是非西班牙裔白人女性中进行的。在这里，我们研究了 CLS-B 和 IL 是否与非裔美国（AA）女性的浸润性 BrCa 相关。
我们评估了来自三个 5 岁年龄匹配组的乳腺活检：来自 Komen 正常组织库（KTB）的无 BrCa 的 AA 女性（50 名志愿者）和来自我们底特律队列的因临床指征进行活检且诊断为良性乳腺疾病（BBD）但后来发展为 BrCa 的 AA 女性（55 名 BBD-癌症）或未发展为 BrCa 的 AA 女性（47 名仅 BBD，活检年份与 BBD-癌症相匹配）。使用 ImageJ 中的 Adiposoft 插件从数字图像中估计脂肪细胞直径和总脂肪面积。使用多变量多项和条件逻辑回归模型评估 KTB 和底特律活检中 CLS-B、IL 与 BrCa 之间的关联。
在所有活检中，在调整了对数脂肪细胞面积、脂肪细胞直径和 BMI 后，志愿者和仅 BBD 活检的 CLS-B 或 IL 的发生率没有显著差异。在临床上指示的 BBD 活检中，与仅 BBD 活检相比，BBD-癌症活检更有可能出现 CLS-B（比值比（OR）=3.36，95%置信区间（CI）：1.33-8.48）或 IL（OR = 4.95，95% CI 1.76-13.9），调整了总脂肪细胞面积、脂肪细胞直径、增殖性疾病和 BMI 后。
CLS-B 和 IL 可能作为 AA 女性良性乳腺活检中 BrCa 风险的组织学标志物。
