Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599, USA.
Department of Pathology and Laboratory Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Clinical Microbiology Laboratory, UNC Medical Center, Chapel Hill, NC 27599, USA.
Cell Rep. 2020 Nov 3;33(5):108352. doi: 10.1016/j.celrep.2020.108352. Epub 2020 Oct 20.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is constantly evolving. Prior studies focused on high-case-density locations, such as the northern and western metropolitan areas of the United States. This study demonstrates continued SARS-CoV-2 evolution in a suburban southern region of the United States by high-density amplicon sequencing of symptomatic cases. 57% of strains carry the spike D614G variant, which is associated with higher genome copy numbers, and its prevalence expands with time. Four strains carry a deletion in a predicted stem loop of the 3' UTR. The data are consistent with community spread within local populations and the larger continental United States. The data instill confidence in current testing sensitivity and validate "testing by sequencing" as an option to uncover cases, particularly nonstandard coronavirus disease 2019 (COVID-19) clinical presentations. This study contributes to the understanding of COVID-19 through an extensive set of genomes from a non-urban setting and informs vaccine design by defining D614G as a dominant and emergent SARS-CoV-2 isolate in the United States.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）在不断进化。先前的研究集中在高病例密度的地区，如美国北部和西部地区。本研究通过对症状性病例进行高密度扩增子测序，展示了美国南部一个郊区的持续 SARS-CoV-2 进化。57%的菌株携带与更高基因组拷贝数相关的刺突 D614G 变异，其流行范围随时间扩大。有 4 株在 3'UTR 的预测茎环中携带缺失。这些数据与当地人群和更大的美国大陆内部的社区传播一致。这些数据增强了对当前检测敏感性的信心，并验证了“通过测序进行检测”作为发现病例的一种选择，特别是对于非标准的 2019 年冠状病毒病（COVID-19）临床表现。本研究通过来自非城市环境的广泛基因组数据集，加深了对 COVID-19 的理解，并通过将 D614G 定义为美国 SARS-CoV-2 分离株中的优势和新兴株，为疫苗设计提供了信息。
Zotero 插件
