Wound Healing Effects of : In Vitro Investigations Using Immortalized Human Keratinocytes (HaCaT).

The traditional use of spp. for the purpose of wound healing has a long history and is widespread internationally. Recently, a hybrid aloe plant () has been cultivated by cross pollination between and . The plant has not yet been investigated for medicinal properties and provides an opportunity for potential biological activity, including wound healing. The aim of this study was to investigate the in vitro wound healing effects of both gel and whole leaf material with the use of the immortalized human keratinocyte (HaCaT) cell line. Cell viability was conducted using methyl thiazolyl tetrazolium (MTT) assays. In vitro wound healing was tested on HaCaT cells using an established scratch assay method. The effect of gel material on HaCaT cell migration was also investigated. gel material exhibited statistically significantly ( < 0.05) higher percentage wound closure compared to the control at all three concentrations investigated. These findings confirm that this newly cultivated species, , also possesses wound healing activity corresponding to that reported for the two species it is derived from, namely, and . Therefore, has the potential to be used in future wound therapeutics.