Pollock H G, Kimmel J R, Hamilton J W, Rouse J B, Ebner K E, Lance V, Rawitch A B
Veterans Administration Medical Center, Kansas City, Missouri 64128.
Gen Comp Endocrinol. 1987 Sep;67(3):375-82. doi: 10.1016/0016-6480(87)90192-4.
Insulin and a 36-residue peptide with homology to pancreatic polypeptide (PP) were isolated from the endocrine pancreas of the alligator gar (Lepisosteus spatula), a ganoid fish, by gel filtration and HPLC. Heterologous radioimmunoassays were used to detect insulin-like and PP-like immunoreactivities during purification of the two peptides. The sequence of the 36-amino acid peptide containing a C-terminal tyrosinamide was identical at 31 of 36 positions to porcine neuropeptide Y (NPY). The amino acid sequence of this peptide is YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH2. The second peptide, gar insulin, contains 52 amino acid residues and is composed of a 21-residue A chain and a 31-residue B chain. The sequence of the A chain is GIVEQCCHKPCTIYELENYCN. The sequence of the B chain is AANQHLCGSHLVEALYLVCGEKGFFYNPNKV.
通过凝胶过滤和高效液相色谱法，从雀鳝（Lepisosteus spatula，一种硬鳞鱼）的内分泌胰腺中分离出胰岛素和一种与胰多肽（PP）具有同源性的36个氨基酸残基的肽。在这两种肽的纯化过程中，使用异源放射免疫分析法检测胰岛素样和PP样免疫反应性。这种含有C端酪氨酰胺的36个氨基酸肽的序列在36个位置中的31个与猪神经肽Y（NPY）相同。该肽的氨基酸序列为YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY-NH2。第二种肽，雀鳝胰岛素，含有52个氨基酸残基，由一个21个氨基酸残基的A链和一个31个氨基酸残基的B链组成。A链的序列为GIVEQCCHKPCTIYELENYCN。B链的序列为AANQHLCGSHLVEALYLVCGEKGFFYNPNKV。
