Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan, China.
J Appl Microbiol. 2021 Aug;131(2):647-657. doi: 10.1111/jam.14912. Epub 2021 Jan 5.
This study aimed to investigate the antibacterial ability and action mechanism of dithiocyano-methane against Aeromonas hydrophila, so as to provide a reference for its application in farm disinfection.
After exposing the bacteria to dithiocyano-methane, the minimum inhibitory concentration (MIC), minimum bactericide concentration (MBC), activities of alkaline phosphatase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase and electric conductivity in bacterial suspensions were determined, transmission electron microscope images on cellular structure and SDS-PAGE profile of bacterial proteins were analysed and the expression of genes related to the above experimental observations was confirmed by real-time quantitative PCR. The MIC and MBC of dithiocyano-methane against three tested strains was 1·46 and 2·93 mg l respectively. The results showed that dithiocyano-methane significantly damaged bacterial cell structure, inhibited the biosynthesis of bacterial proteins and changed the integrity and permeability of bacterial cell wall and cell membrane.
Dithiocyano-methane showed remarkable antibacterial ability against three tested strains, indicating it is a potential effective bactericidal agent for preventing animal diseases resulted from Aer. hydrophila.
To our best knowledge, this is the first report to examine the antibacterial ability and action mechanism of dithiocyano-methane against bacteria. The results demonstrate the great potential of dithiocyano-methane as a disinfectant against Aer. hydrophila in settings such as aquaculture ponds and livestock farms.
本研究旨在探讨二硫氰基甲烷对嗜水气单胞菌的抗菌能力和作用机制，为其在养殖场消毒中的应用提供参考。
将细菌暴露于二硫氰基甲烷后，测定最低抑菌浓度（MIC）、最低杀菌浓度（MBC）、碱性磷酸酶、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、乳酸脱氢酶活性和细菌悬浮液的电导率，分析细胞结构的透射电镜图像和细菌蛋白的 SDS-PAGE 图谱，并通过实时定量 PCR 确认与上述实验观察相关的基因表达。二硫氰基甲烷对 3 株受试菌的 MIC 和 MBC 分别为 1.46 和 2.93 mg l。结果表明，二硫氰基甲烷显著破坏了细菌的细胞结构，抑制了细菌蛋白质的合成，并改变了细菌细胞壁和细胞膜的完整性和通透性。
二硫氰基甲烷对 3 株受试菌表现出显著的抗菌能力，表明它是一种预防由嗜水气单胞菌引起的动物疾病的潜在有效杀菌剂。
据我们所知，这是首次研究二硫氰基甲烷对细菌的抗菌能力和作用机制。研究结果表明，二硫氰基甲烷作为水产养殖池塘和养殖场中防治嗜水气单胞菌的消毒剂具有很大的潜力。
