Chen Yanfeng, Ke Wenjie, Qin Huabin, Chen Siwei, Qin Limei, Yang Ying, Yu Hui, Tan Yuansheng
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes, School of Life Science and Engineering, Foshan University, Foshan, 528231, China.
AMB Express. 2020 Nov 11;10(1):205. doi: 10.1186/s13568-020-01142-z.
This paper studied the inhibitory effects of dithiocyano-methane (DM) on the glucose decomposition pathway in the respiratory metabolism of Escherichia coli. We investigated the effects of DM on the activities of key enzymes (ATPase and glucose-6-phosphate dehydrogenase, G6PDH), the levels of key product (nicotinamide adenosine denucleotide hydro-phosphoric acid, NADPH), and gene expression in the hexose monophosphate pathway (HMP). The results showed that the minimum inhibitory concentration (MIC) and the minimum bactericide concentration (MBC) of DM against the tested strains were 5.86 mg/L and 11.72 mg/L, respectively. Bacteria exposed to DM at MIC demonstrated an increase in bacterial ATPase and G6PDH activities, NADPH levels, and gene expression in the HMP pathway compared to bacteria in the control group, which could be interpreted as a behavioral response to stress introduced by DM. However, DM at a lethal concentration of 10 × MIC affected glucose decomposition by inhibiting mainly the HMP pathway in E. coli.
本文研究了二硫氰基甲烷（DM）对大肠杆菌呼吸代谢中葡萄糖分解途径的抑制作用。我们研究了DM对关键酶（ATP酶和葡萄糖-6-磷酸脱氢酶，G6PDH）活性、关键产物（烟酰胺腺嘌呤二核苷酸磷酸，NADPH）水平以及磷酸戊糖途径（HMP）中基因表达的影响。结果表明，DM对受试菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为5.86 mg/L和11.72 mg/L。与对照组细菌相比，暴露于MIC浓度DM下的细菌其ATP酶和G6PDH活性、NADPH水平以及HMP途径中的基因表达均有所增加，这可被解释为对DM引入的应激的行为反应。然而，致死浓度为10×MIC的DM主要通过抑制大肠杆菌的HMP途径来影响葡萄糖分解。
