Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Plant Physiol Biochem. 2020 Dec;157:185-194. doi: 10.1016/j.plaphy.2020.10.025. Epub 2020 Oct 23.
The frequency and severity of global abiotic stresses such as heat, drought, and salt stress are increasing due to climate changes. Objectives of this study were to investigate effects of γ-aminobutyric acid (GABA) priming on inducing plants' acclimation to abiotic stress associated with alterations of endogenous polyamines (PAs), amino acids, and sugars in creeping bentgrass (Agrostis stolonifera). The pretreatment with GABA fertigation significantly alleviated heat-, drought-, and salt-induced declines in leaf relative water content, chlorophyll content, cell membrane stability, photochemical efficiency (Fv/Fm), and performance index on absorption basis (PIABS), and also further decreased stress-caused decline in osmotic potential in leaves. The GABA priming uniformly increased total PAs, spermidine, amino acids involved in GABA shunt (GABA, glutamic acid, and alanine), and other amino acids (phenylalanine, aspartic acid, and glycine) accumulation under heat, drought, and salt stress. The GABA priming also significantly improved methionine content under heat and drought stress, maltose, galactose, and talose content under heat and salt stress, or cysteine, serine, and threonine content under drought and salt stress. Interestingly, the GABA priming uniquely led to significant accumulation of spermine, fructose, and glucose under heat stress, putrescine, proline, and mannose under drought stress, or arginine, trehalose and xylose under salt stress, respectively. These particular PAs, sugars, and amino acids differentially or commonly regulated by GABA could play critical roles in osmotic adjustment, osmoprotection, antioxidant, energy source, and signal molecular for creeping bentgrass to acclimate diverse abiotic stresses.
由于气候变化，全球非生物胁迫（如热胁迫、干旱胁迫和盐胁迫）的频率和严重程度正在增加。本研究的目的是研究γ-氨基丁酸（GABA）引发处理对提高匍匐翦股颖（Agrostis stolonifera）对与内源多胺（PAs）、氨基酸和糖变化相关的非生物胁迫的适应能力的影响。GABA 施肥预处理显著缓解了热胁迫、干旱胁迫和盐胁迫导致的叶片相对含水量、叶绿素含量、细胞膜稳定性、光化学效率（Fv/Fm）和基于吸收的性能指数（PIABS）下降，也进一步降低了叶片胁迫引起的渗透势下降。GABA 引发处理均匀增加了总多胺、腐胺、GABA 分流（GABA、谷氨酸和丙氨酸）和其他氨基酸（苯丙氨酸、天冬氨酸和甘氨酸）在热胁迫、干旱胁迫和盐胁迫下的积累。GABA 引发处理还显著提高了热胁迫和干旱胁迫下蛋氨酸、热胁迫和盐胁迫下麦芽糖、半乳糖和塔罗糖、干旱胁迫和盐胁迫下胱氨酸、丝氨酸和苏氨酸的含量。有趣的是，GABA 引发处理在热胁迫下独特地导致了腐胺、果糖和葡萄糖的显著积累，在干旱胁迫下导致了精胺、脯氨酸和甘露糖的积累，在盐胁迫下导致了精氨酸、海藻糖和木糖的积累。这些由 GABA 差异化或共同调节的特定 PAs、糖和氨基酸可能在渗透调节、渗透保护、抗氧化、能量来源和信号分子方面对匍匐翦股颖适应多种非生物胁迫发挥关键作用。
