Department of Genetic and Plant Production, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Crop and Horticultural Science Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Moghan, Iran.
Sci Rep. 2022 Aug 27;12(1):14648. doi: 10.1038/s41598-022-18229-1.
The effect of triad application of the phytohormone 24-epibrassinolide (EBL), the polyamine spermine (Spm), and the element silicon (Si) has not yet been considered on plant growth and behavior in water-stressed conditions. We aimed to evaluate the impact of single/dual/triad application of 24-epibrassinolide (EBL), spermine (Spm), and silicon (Si) on the growth, photosynthetic metabolites, and antioxidant enzymes in the maize plant exposed to water stress. This study was conducted as a potential drought resistance system and plants' maintenance against oxidative damage. In this regard, one maize hybrid (Paya) was grown under well-watered and water-deficit conditions (interrupted irrigation at the flowering and the filling seed stages) with and without foliar spraying of EBL, Spm, and/or Si. Drought conditions remarkably reduced growth, productivity, water-related content (RWC), and chlorophyll content. However, the dual and triad applications of EBL (0.1 mg L), Spm (25 mg L), and Si (7 mg L) significantly improved the above parameters. Water stress considerably augmented the levels of HO and MDA. Their content in stress-subjected plants was significantly reduced by triad application. In water-stressed circumstances and after foliar treatments, the activities of superoxide dismutase, catalase, and peroxidase as well as the amounts of total soluble proteins, phenolic compounds, proline, and glycine betaine all improved. Overall, triad application increased the plant's drought resistance and diminished ROS accumulation by raising the scavenging via the enhanced activity of the antioxidant enzymes.
三组分应用植物激素 24-表油菜素内酯（EBL）、多胺精胺（Spm）和硅（Si）对植物在胁迫条件下的生长和行为的影响尚未被考虑。我们旨在评估单一/双重/三重应用 24-表油菜素内酯（EBL）、精胺（Spm）和硅（Si）对暴露于水分胁迫下玉米植株生长、光合代谢物和抗氧化酶的影响。本研究作为一种潜在的抗旱系统和植物对氧化损伤的维持进行了研究。为此，在充分浇水和缺水条件下（开花和灌浆种子阶段中断灌溉），对一个玉米杂种（Paya）进行了研究，并进行了叶面喷施 EBL、Spm 和/或 Si。干旱条件显著降低了生长、生产力、水分含量（RWC）和叶绿素含量。然而，EBL（0.1mg/L）、Spm（25mg/L）和 Si（7mg/L）的双重和三重应用显著改善了上述参数。水分胁迫显著增加了 HO 和 MDA 的含量。在胁迫下，三组分应用显著降低了它们的含量。在水分胁迫和叶面处理后，超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性以及总可溶性蛋白质、酚类化合物、脯氨酸和甘氨酸甜菜碱的含量均有所提高。总的来说，三组分应用通过提高抗氧化酶的活性来增加植物的抗旱性和减少 ROS 积累。
