Dong Yalan, Li Jianfeng, Liu Rongju, Zhao Zhenxiang, Wang Shuaibin, Cui Kai
Department of Neurosurgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei China.
Medicine (Baltimore). 2020 Oct 23;99(43):e22000. doi: 10.1097/MD.0000000000022000.
The expression of tumor stem cell markers musashi1 (msi1) and numb in brain metastases were detected to explore their roles in the development of brain metastases.A total of 51 cases of brain metastasis, 29 cases of primary tumor and 15 cases of normal brain tissue were selected. Immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) were used to detect msi1 and numb expression at the protein and mRNA levels. Correlation between msi1 and numb in brain metastases were evaluated.Immunohistochemistry and RT-PCR showed that no significant difference in the expression of msi1 and numb between brain metastases and primary tumors was observed (P > .05); the expression of msi1 and numb in brain metastases was significantly higher than that in normal brain tissues (P < .05); and the expression of msi1 and numb in primary tumors was significantly higher than that in normal brain tissues (P < .05). In general, the expression of msi1 gene was negatively correlated with the expression of numb at mRNA level by Pearson correlation analysis (r = -0.345, P < .05). Additionally, the expression of msi1 and numb in brain metastases was not related to gender, age, and tissue origin (P > .05).Msi1 is highly expressed in brain metastases and primary tumors, while numb is lowly expressed in brain metastases and primary tumors; msi1 and numb are negatively correlated in brain metastases, suggesting that msi1 and numb may have regulatory mechanisms in the development of brain metastases.
检测脑转移瘤中肿瘤干细胞标志物musashi1（msi1）和numb的表达，以探讨它们在脑转移瘤发生发展中的作用。共选取51例脑转移瘤、29例原发肿瘤和15例正常脑组织。采用免疫组织化学和逆转录聚合酶链反应（RT-PCR）检测msi1和numb在蛋白质和mRNA水平的表达。评估脑转移瘤中msi1和numb之间的相关性。免疫组织化学和RT-PCR结果显示，脑转移瘤和原发肿瘤中msi1和numb的表达无显著差异（P>0.05）；脑转移瘤中msi1和numb的表达显著高于正常脑组织（P<0.05）；原发肿瘤中msi1和numb的表达显著高于正常脑组织（P<0.05）。总体而言，经Pearson相关性分析，msi1基因在mRNA水平的表达与numb的表达呈负相关（r=-0.345，P<0.05）。此外，脑转移瘤中msi1和numb的表达与性别、年龄和组织来源无关（P>0.05）。Msi1在脑转移瘤和原发肿瘤中高表达，而numb在脑转移瘤和原发肿瘤中低表达；脑转移瘤中msi1和numb呈负相关，提示msi1和numb可能在脑转移瘤的发生发展中具有调控机制。
