Juang Yi-Je, Wang Yu, Hsu Shu-Kai
Department of Chemical Engineering, National Cheng Kung University, No.1 University Road, Tainan 70101, Taiwan.
Center for Micro/nano Science and Technology, National Cheng Kung University, No.1 University Road, Tainan 70101, Taiwan.
Polymers (Basel). 2020 Oct 27;12(11):2493. doi: 10.3390/polym12112493.
In recent years, microfluidic paper-based analytical devices (µPADs) have been developed because they are simple, inexpensive and power-free for low-cost chemical, biological and environmental detection. Moreover, paper is lightweight; easy to stack, store and transport; biodegradable; biocompatible; good for colorimetric tests; flammable for easy disposal of used paper-based diagnostic devices by incineration; and can be chemically modified. Different methods have been demonstrated to fabricate µPADs such as solid wax printing, craft cutting, photolithography, etc. In this study, one-step hot microembossing was proposed and demonstrated to fabricate µPADs. The processing parameters like embossing temperature, pressure and time were systematically investigated. It was found that, at 55 °C embossing temperature, the embossing pressure ranging from 10 to 14 MPa could be applied and the embossing time was only 5 s. This led to the overall processing time for fabrication of µPADs within 10 s. Glucose detection was conducted using the µPADs as fabricated, and a linear relationship was obtained between 5 and 50 mM.
近年来，基于微流控纸的分析装置（µPADs）得以开发，因为它们简单、廉价且无需电源，适用于低成本的化学、生物和环境检测。此外，纸张重量轻；易于堆叠、存储和运输；可生物降解；具有生物相容性；适用于比色测试；可燃，便于通过焚烧轻松处置用过的纸质诊断装置；并且可以进行化学改性。已经展示了不同的方法来制造µPADs，如固体蜡印刷、手工切割、光刻等。在本研究中，提出并展示了一步热微压印来制造µPADs。系统研究了压印温度、压力和时间等工艺参数。结果发现，在55°C的压印温度下，可以施加10至14MPa的压印压力，压印时间仅为5秒。这使得制造µPADs的总处理时间在10秒以内。使用制造好的µPADs进行了葡萄糖检测，在5至50mM之间获得了线性关系。
