Tuomanen E, Cozens R
Rockefeller University, New York, New York 10021.
J Bacteriol. 1987 Nov;169(11):5308-10. doi: 10.1128/jb.169.11.5308-5310.1987.
The composition of peptidoglycan of chemostat-grown cultures of Escherichia coli was investigated as a function of growth rate. As the generation time was lengthened from 0.8 to 13.8 h, there was a decrease in the major monomer (disaccharide tetrapeptide) and dimer (bis-disaccharide tetrapeptide), while disaccharide tripeptide moieties increased to greater than 50% of the total wall. The average chain length became much shorter; lipoprotein density tripled, and the number of unusual diaminopimelyl-diaminopimelic acid crossbridges increased fivefold. As cells grew more slowly, amounts of penicillin-binding proteins (PBPs) 1a-1b complex and 4 decreased, while amounts of PBPs 3 and the 5-6 complex increased. We propose that the chemical composition of E. coli cell walls changes with growth rate in a manner consistent with alterations in the activities of PBPs and cell shape.
研究了恒化器培养的大肠杆菌中肽聚糖的组成随生长速率的变化情况。随着代时从0.8小时延长至13.8小时，主要单体（二糖四肽）和二聚体（双二糖四肽）减少，而二糖三肽部分增加至占总细胞壁的50%以上。平均链长变得更短；脂蛋白密度增加了两倍，异常的二氨基庚二酸-二氨基庚二酸交联桥数量增加了五倍。随着细胞生长变慢，青霉素结合蛋白（PBPs）1a-1b复合物和4的量减少，而PBPs 3和5-6复合物的量增加。我们提出，大肠杆菌细胞壁的化学成分随生长速率而变化，其方式与PBPs活性和细胞形状的改变一致。
