Mohajerani Hassan, Irajian Gholamreza, Latifi Fatemeh, Masjedian Faramarz, Tabrizi Reza
Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran.
Int J Implant Dent. 2020 Oct 30;6(1):70. doi: 10.1186/s40729-020-00263-1.
Clindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells. The aim of this study was to evaluate the efficacy of local clindamycin (20 μg/mL) in two different exposure times, for microbial decontamination of particulate bone graft, collected during implant site preparation. This non-randomized parallel-group study was conducted on samples from 17 patients. The particulate bone collected during implant site preparation was divided into three portions by weight: in group S1, the particulate bone was immersed in thioglycolate broth without any antibiotic treatment; in group S2, the collected particulate bone was irrigated with 100 mL clindamycin solution (20 μg/mL); and in group S3, the collected particulate bone was soaked in one ml clindamycin solution (20 μg/mL) for 3 min. Samples in the three groups were cultured in aerobic and anaerobic media and species and CFU count of isolated bacteria were determined.
Analysis of the data demonstrated a significant difference among the three groups in the mean count of total microorganisms (P = 0.001). The difference in the mean count of anaerobic and aerobic microorganisms in the three groups was statistically significant as well (P = 0.001). Pseudomonas aeruginosa was the only microorganism that was not affected with the mentioned antibiotic.
Local use of low-dose clindamycin (20 μg/mL)-irrigation or 3 min immersing-is effective for the decontamination of particulate bone grafts.
低浓度（20μg/mL）的克林霉素对骨细胞的活力和成骨潜能是安全的。本研究的目的是评估局部应用克林霉素（20μg/mL）在两种不同暴露时间下，对种植位点预备过程中采集的颗粒状骨移植材料进行微生物去污的效果。这项非随机平行组研究对17例患者的样本进行。将种植位点预备过程中采集的颗粒状骨按重量分成三份：在S1组，颗粒状骨浸入硫乙醇酸盐肉汤中，未进行任何抗生素处理；在S2组，采集的颗粒状骨用100mL克林霉素溶液（20μg/mL）冲洗；在S3组，采集的颗粒状骨在1mL克林霉素溶液（20μg/mL）中浸泡3分钟。三组样本在需氧和厌氧培养基中培养，并测定分离细菌的种类和菌落形成单位数量。
数据分析表明，三组之间总微生物平均计数存在显著差异（P = 0.001）。三组中厌氧和需氧微生物平均计数的差异也具有统计学意义（P = 0.001）。铜绿假单胞菌是唯一不受上述抗生素影响的微生物。
局部使用低剂量克林霉素（20μg/mL）冲洗或浸泡3分钟对颗粒状骨移植材料的去污是有效的。
