Mahajan Shifali, Kaur Rose Kanwaljeet, Grover Vishakha, Mehta Manjula, Prashar Savita, Jain Ashish
Department of Periodontics, Yamuna Institute of Dental Sciences and Research, Yamuna Nagar, Haryana, India.
Department of Periodontics, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Punjab University, Chandigarh, India.
J Indian Soc Periodontol. 2024 Nov-Dec;28(6):673-679. doi: 10.4103/jisp.jisp_19_24. Epub 2025 Apr 3.
The efficacy of autogenous tooth bone graft (auto-TBG) is affected by many determinants, such as sterilization protocols and the expression of bone-inducing proteins such as bone morphogenetic protein (BMP) present in the graft.
This study was conducted to compare the efficacy of different sterilization protocols and their impact on the osteogenic potential of auto-TBG.
Ninety-eight auto-TBG samples were obtained from 10 extracted teeth that were prepared, ground, and sorted with the help of a dentin grinder. Seventy samples were partially demineralized with the standard recommended techniques, and 28 samples were kept nondemineralized (ND) for comparison. All the processed samples were subjected to different sterilization protocols namely Group A (gamma radiation), Group B (autoclave), Group C (chemical sterilization): Subgroup a (chlorhexidine), Subgroup b (povidone-iodine), Subgroup c (lactic acid), Subgroup d (sodium hydroxide), and Group D (normal saline). Sterilized auto-TBG samples were subjected to microbiological and biochemical analysis.
All sterilization protocols could effectively eliminate the microbial growth except normal saline. Mean BMP-2 concentration (pg/mL) was found as 141.91 (chlorhexidine) >134.50 (sodium hydroxide) >134.17 (lactic acid) >133.83 (povidone-iodine) >122.83 (gamma radiation) >121.81 (autoclave) >119.80 (normal saline).
Comparatively, the chemical method of sterilization showed a higher mean concentration of BMP-2. In addition, all the samples of demineralized auto-TBG particles showed significantly higher mean BMP-2 concentration as compared to ND samples.
自体牙骨移植（auto-TBG）的疗效受多种因素影响，如灭菌方案以及移植体中存在的骨诱导蛋白（如骨形态发生蛋白（BMP））的表达。
本研究旨在比较不同灭菌方案的疗效及其对auto-TBG成骨潜能的影响。
从10颗拔除的牙齿中获取98个auto-TBG样本，借助牙本质研磨机进行制备、研磨和分类。70个样本采用标准推荐技术进行部分脱矿，28个样本保持未脱矿（ND）作为对照。所有处理后的样本接受不同的灭菌方案，即A组（γ射线辐射）、B组（高压灭菌）、C组（化学灭菌）：a亚组（洗必泰）、b亚组（聚维酮碘）、c亚组（乳酸）、d亚组（氢氧化钠），以及D组（生理盐水）。对灭菌后的auto-TBG样本进行微生物学和生化分析。
除生理盐水外，所有灭菌方案均能有效消除微生物生长。发现平均BMP-2浓度（pg/mL）为141.91（洗必泰）>134.50（氢氧化钠）>134.17（乳酸）>133.83（聚维酮碘）>122.83（γ射线辐射）>121.81（高压灭菌）>119.80（生理盐水）。
相比之下，化学灭菌方法显示出较高的平均BMP-2浓度。此外，与ND样本相比，所有脱矿的auto-TBG颗粒样本的平均BMP-2浓度均显著更高。
