Gullstrand Lab., Ophthalmology, Dept. of Neuroscience, Uppsala University, Sweden.
Exp Eye Res. 2013 Aug;113:26-31. doi: 10.1016/j.exer.2013.04.015. Epub 2013 May 1.
The purpose of this study was to investigate if topically applied caffeine protects against in vivo ultraviolet radiation cataract and if so, to estimate the protection factor. Three experiments were carried out. First, two groups of Sprague-Dawley rats were pre-treated with a single application of either placebo or caffeine eye drops in both eyes. All animals were then unilaterally exposed in vivo to 8 kJ/m(2) UV-B radiation for 15 min. One week later, the lens GSH levels were measured and the degree of cataract was quantified by measurement of in vitro lens light scattering. In the second experiment, placebo and caffeine pre-treated rats were divided in five UV-B radiation dose groups, receiving 0.0, 2.6, 3.7, 4.5 or 5.2 kJ/m(2) UV-B radiation in one eye. Lens light scattering was determined after one week. In the third experiment, placebo and caffeine pre-treated rats were UV-B-exposed and the presence of activated caspase-3 was visualized by immunohistochemistry. There was significantly less UV-B radiation cataract in the caffeine group than in the placebo group (95% confidence interval for mean difference in lens light scattering between the groups = 0.10 ± 0.05 tEDC), and the protection factor for caffeine was 1.23. There was no difference in GSH levels between the placebo- and the caffeine group. There was more caspase-3 staining in UV-B-exposed lenses from the placebo group than in UV-B-exposed lenses from the caffeine group. Topically applied caffeine protects against ultraviolet radiation cataract, reducing lens sensitivity 1.23 times.
本研究旨在探讨局部应用咖啡因是否能预防体内紫外线辐射白内障，如果能，那么估计其保护因子。进行了三项实验。首先，两组 Sprague-Dawley 大鼠双眼分别用安慰剂或咖啡因眼药水单次预处理。然后所有动物单侧接受 8 kJ/m(2) 的 UV-B 辐射 15 分钟。一周后，测量晶状体 GSH 水平，并通过测量体外晶状体光散射来量化白内障程度。在第二项实验中，将用安慰剂和咖啡因预处理的大鼠分为 5 个 UV-B 辐射剂量组，每组一只眼接受 0.0、2.6、3.7、4.5 或 5.2 kJ/m(2) 的 UV-B 辐射。一周后测定晶状体光散射。在第三项实验中，用安慰剂和咖啡因预处理的大鼠接受 UV-B 照射，并用免疫组织化学法观察激活的半胱氨酸天冬氨酸蛋白酶-3 的存在。与安慰剂组相比，咖啡因组的紫外线辐射白内障明显减少（组间晶状体光散射平均差异的 95%置信区间=0.10 ± 0.05 tEDC），咖啡因的保护因子为 1.23。安慰剂组和咖啡因组之间的 GSH 水平没有差异。与咖啡因组相比，来自安慰剂组的 UV-B 照射的晶状体中 caspase-3 染色更多。局部应用咖啡因可预防紫外线辐射白内障，使晶状体敏感性降低 1.23 倍。
