Liu Zening, Cui Jinyan, Zhan Wei
Department of Chemistry and Biochemistry, Auburn University, Auburn, Alabama 36849, United States.
Langmuir. 2020 Nov 10;36(44):13193-13200. doi: 10.1021/acs.langmuir.0c01496. Epub 2020 Oct 30.
Combining gel-assisted lipid hydration with membrane-based lipid extrusion, we demonstrate here a general procedure for rapid preparation of giant unilamellar liposomes with upper size control. Featured in this procedure are planar lipid stacks deposited on poly(vinyl alcohol) gel, which are further laminated atop with microporous polycarbonate membranes. Control of liposome size is thus realized through the uniform-sized pores of the latter, which provide the only access for the underlying lipids to enter the main aqueous phase upon hydration. Production of both single-phased and biphasic (Janus) liposomes using several commonly employed model lipids, including 1,2-dipalmitoyl--glycero-3-phosphocholine (DPPC), 1,2-dioleoyl--glycero-3-phosphocholine (DOPC), 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and cholesterol, is presented. The size distribution, yield and lamellarity of these liposome products are characterized and analyzed in detail by confocal fluorescence microscopy. This procedure thus offers a simple and fast alternative route to giant unilamellar liposomes with upper size control.
将凝胶辅助脂质水合与基于膜的脂质挤出相结合，我们在此展示了一种快速制备具有上限尺寸控制的巨型单层脂质体的通用方法。该方法的特点是将平面脂质堆叠沉积在聚乙烯醇凝胶上，然后在其上进一步层压微孔聚碳酸酯膜。脂质体尺寸的控制是通过后者均匀尺寸的孔实现的，这些孔是下层脂质在水合时进入主要水相的唯一通道。使用几种常用的模型脂质，包括1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）、1,2-二油酰-sn-甘油-3-磷酸胆碱（DOPC）、1-棕榈酰-2-油酰-甘油-3-磷酸胆碱（POPC）和胆固醇，制备了单相和双相（Janus）脂质体。通过共聚焦荧光显微镜对这些脂质体产品的尺寸分布、产率和层数进行了详细的表征和分析。因此，该方法为制备具有上限尺寸控制的巨型单层脂质体提供了一种简单快速的替代途径。
