Key Laboratory of Inshore Resources Biotechnology (Quanzhou Normal University), Fujian Province University, Quanzhou 362000, China.
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Molecules. 2021 Mar 4;26(5):1385. doi: 10.3390/molecules26051385.
Marine collagen peptides have high potential in promoting skin wound healing. This study aimed to investigate wound healing activity of collagen peptides derived from (SNCP). The effects of SNCP on promoting healing were studied through a whole cortex wound model in mice. Results showed that SNCP consisted of peptides with a molecular weight less than 5 kDa accounted for 81.95%, rich in Gly and Arg. SNCP possessed outstanding capacity to induce human umbilical vein endothelial cells (HUVEC), human immortalized keratinocytes (HaCaT) and human skin fibroblasts (HSF) cells proliferation and migration in vitro. In vivo, SNCP could markedly improve the healing rate and shorten the scab removal time, possessing a scar-free healing effect. Compared with the negative control group, the expression level of tumor necrosis factor-α, interleukin-1β and transforming growth factor-β1 (TGF-β1) in the SNCP group was significantly down-regulated at 7 days post-wounding ( < 0.01). Moreover, the mRNA level of mothers against decapentaplegic homolog 7 (Smad7) in SNCP group was up-regulated ( < 0.01); in contrast, type II TGF-β receptors, collagen I and α-smooth muscle actin were significantly down-regulated at 28 days ( < 0.01). These results indicate that SNCP possessed excellent activity of accelerating wound healing and inhibiting scar formation, and its mechanism was closely related to reducing inflammation, improving collagen deposition and recombination and blockade of the TGF-β/Smads signal pathway. Therefore, SNCP may have promising clinical applications in skin wound repair and scar inhibition.
海洋胶原蛋白肽在促进皮肤伤口愈合方面具有很大的潜力。本研究旨在研究来源于 （SNCP）的胶原蛋白肽的伤口愈合活性。通过小鼠全皮层伤口模型研究了 SNCP 对促进愈合的作用。结果表明，SNCP 由分子量小于 5 kDa 的肽组成，占 81.95%，富含甘氨酸和精氨酸。SNCP 具有出色的诱导人脐静脉内皮细胞（HUVEC）、人永生化角质形成细胞（HaCaT）和人皮肤成纤维细胞（HSF）细胞体外增殖和迁移的能力。在体内，SNCP 可显著提高愈合率并缩短结痂去除时间，具有无疤痕愈合的效果。与阴性对照组相比，SNCP 组在创伤后 7 天 TNF-α、IL-1β 和转化生长因子-β1（TGF-β1）的表达水平显著下调（ < 0.01）。此外，SNCP 组的 Mothers against decapentaplegic homolog 7（Smad7）mRNA 水平上调（ < 0.01）；相反，28 天时 II 型 TGF-β 受体、I 型胶原和α-平滑肌肌动蛋白显著下调（ < 0.01）。这些结果表明，SNCP 具有优异的加速伤口愈合和抑制疤痕形成的活性，其机制与减少炎症、改善胶原沉积和重组以及阻断 TGF-β/Smads 信号通路密切相关。因此，SNCP 可能在皮肤伤口修复和抑制疤痕方面具有广阔的临床应用前景。
