Drexler H G, Amlot P L, Minowada J
Department of Haematology, Royal Free Hospital School of Medicine, London, UK.
Leukemia. 1987 Sep;1(9):629-37.
The origin of Hodgkin (H) and Reed-Sternberg (RS) cells remains a highly controversial issue. Studies on noncultured, freshly disaggregated biopsy material or of histological sections have not definitely established the cell lineage of HD cells, although evidence has been gathered that has allowed interpretation to favor one or the other hematopoietic cell type. In recent years a number of cell lines have been established from patients with Hodgkin's disease (HD) which are claimed to represent in vitro counterparts of H-RS cells. The phenotypic and functional properties of 7 HD-derived cell lines are reviewed here. The cell lines are not identical; they show many common features, but also a number of important differences in their phenotypes as well as in their in vitro behavior. The cell lines differ with some having characteristics of T cells, others of B cells, and yet others of the monocyte/macrophage cell lineage. This heterogeneity of H-RS established cell lines could be explained in a variety of ways: (a) the heterogeneity of HD itself might result from a disease process which leads to fusion of different cell types resulting in different phenotypic forms of H-RS cells depending on the cells involved; (b) the different cell types might reflect differences in the malignant cells present in the separate subtypes of HD; (c) the difficulty in successfully establishing H-RS cell lines might mean that unrepresentative cell types adapt to in vitro conditions. Rather than resolve the origin of H-RS cells, the established HD-derived cell lines have been consistent with the heterogeneity described in histochemical, immunohistological, and immunocytochemical studies.
霍奇金（H）细胞和里德-斯腾伯格（RS）细胞的起源仍然是一个极具争议的问题。对未经培养、刚解离的活检材料或组织切片的研究尚未明确确定HD细胞的细胞谱系，尽管已收集到的证据使得人们倾向于将其解释为某一种造血细胞类型。近年来，已从霍奇金病（HD）患者中建立了许多细胞系，据称这些细胞系代表了H-RS细胞的体外对应物。本文综述了7种源自HD的细胞系的表型和功能特性。这些细胞系并不完全相同；它们表现出许多共同特征，但在表型以及体外行为方面也存在一些重要差异。这些细胞系各不相同，有些具有T细胞特征，有些具有B细胞特征，还有一些具有单核细胞/巨噬细胞系特征。已建立的H-RS细胞系的这种异质性可以用多种方式来解释：（a）HD本身的异质性可能是由一种疾病过程导致的，该过程导致不同细胞类型融合，根据所涉及的细胞产生不同表型形式的H-RS细胞；（b）不同的细胞类型可能反映了HD不同亚型中存在的恶性细胞的差异；（c）成功建立H-RS细胞系的困难可能意味着非代表性的细胞类型适应了体外条件。已建立的源自HD的细胞系并没有解决H-RS细胞的起源问题，而是与组织化学、免疫组织学和免疫细胞化学研究中描述的异质性相一致。
