Drexler H G, Minowada J
Fujisaki Cell Center, Hayashibara Biochemical Labs., Inc.
Hum Cell. 1992 Mar;5(1):42-53.
A number of so-called "HD cell lines" have been established over the last 10-15 years (Table 1). Or those 15 cell lines we studied, only the cell lines CO, DEV, HD-70, HDLM, KM-H2, L-428, L-540 and SUP-HD1 can be regarded to represent true HD cell lines. According to the immunostaining results and molecular genetic data, these 8 cell lines can be assigned either to the T-cell lineage (CO, HDLM, L-540) or B-cell lineage (DEV, HD-70, KM-H2, SUP-HD1). With the data currently available, the cell lineage origin of L-428 cannot be unequivocally determined, but appears to be lymphoid. All but one of these eight HD cell lines have been established from patients with the nodular sclerosis subtype. Therefore, the conclusions drawn from the in vitro studies are limited to this histological subtype of HD. It is conceivable that culture conditions select for a particular type of cell that will survive. The state of differentiation of these HD cell lines remains unclear due to the incomplete expression of T- or B-cell antigens. The in vitro cells and the in vivo H-RS cells share, however, the expression of the unique activation markers CD15, CD25, CD30, CD71 and HLA-DR. Recently published data indicate that the HD cell lines express and produce a large number of cytokines. Multiple non-random chromosomal abnormalities and the expression of various proto-oncogenes are also new and exciting findings and certainly deserve further study. In summary, although the cultured cells are not unequivocally proven to be the direct progeny of in vivo H-RS cells, several continuous HD cell lines have been established that display a variety of phenotypical features identical or similar to those of their presumed in vivo counterparts. Surface marker, molecular genetic and other features suggest a T- or B-cell derivation. An extrapolation of these conclusions would point to a lymphoid origin of H-RS cells. Whether H-RS cells can originate from other cell types such as monocytes/macrophages or reticulum cells, cannot be answered with the currently available HD cell lines.
在过去10到15年里建立了许多所谓的“霍奇金淋巴瘤细胞系”（表1）。在我们研究的这15个细胞系中，只有CO、DEV、HD - 70、HDLM、KM - H2、L - 428、L - 540和SUP - HD1细胞系可被视为代表真正的霍奇金淋巴瘤细胞系。根据免疫染色结果和分子遗传学数据，这8个细胞系可归为T细胞谱系（CO、HDLM、L - 540）或B细胞谱系（DEV、HD - 70、KM - H2、SUP - HD1）。根据目前可得的数据，L - 428的细胞谱系起源尚不能明确确定，但似乎是淋巴细胞系。这8个霍奇金淋巴瘤细胞系中除一个外均来自结节硬化亚型患者。因此，体外研究得出的结论仅限于霍奇金淋巴瘤的这种组织学亚型。可以想象，培养条件选择了能够存活的特定类型细胞。由于T或B细胞抗原表达不完全，这些霍奇金淋巴瘤细胞系的分化状态仍不清楚。然而，体外细胞和体内霍奇金 - 里德 - 斯腾伯格（H - RS）细胞共同表达独特的活化标志物CD15、CD25、CD30、CD71和HLA - DR。最近发表的数据表明，霍奇金淋巴瘤细胞系表达并产生大量细胞因子。多种非随机染色体异常以及各种原癌基因的表达也是新的、令人兴奋的发现，当然值得进一步研究。总之，尽管培养细胞尚未被明确证明是体内H - RS细胞的直接后代，但已建立了几个连续的霍奇金淋巴瘤细胞系，它们表现出与其假定的体内对应物相同或相似的多种表型特征。表面标志物、分子遗传学和其他特征提示其起源于T或B细胞。这些结论的外推将指向H - RS细胞的淋巴细胞起源。H - RS细胞是否能起源于其他细胞类型，如单核细胞/巨噬细胞或网状细胞，目前的霍奇金淋巴瘤细胞系无法回答。
