Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, University of Toronto, Toronto, ON, Canada; Department of Immunology, University of Toronto, Toronto, ON, Canada.
Toronto General Hospital Research Institute, University Health Network, Toronto, ON, Canada; Translational Biology and Engineering Program, Ted Rogers Centre for Heart Research, University of Toronto, Toronto, ON, Canada; Department of Immunology, University of Toronto, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; Peter Munk Cardiac Centre, Toronto General Hospital, Toronto, ON, Canada.
Curr Opin Immunol. 2021 Feb;68:54-63. doi: 10.1016/j.coi.2020.09.005. Epub 2020 Oct 28.
Cardiac macrophages maintain homeostasis and orchestrate response to disease. Utilization of genetic fate-mapping and single-cell RNA sequencing shifted the paradigm of macrophage heterogeneity from the canonical M1/M2 classification in favour of a nuanced approach that reconciles divergent origins, lifecycles, and transcriptional states. Here, we provide a conceptual framework to assess cardiac macrophage complexity that integrates transcriptional and functional heterogeneity that tracks with subset-specific markers (TIMD4 and CCR2). Our goal is to provide a starting point for researchers to dissect the functions of known resident cardiac macrophage subpopulations. We discuss recent advances and limitations in our understanding of cardiac macrophage diversity in ischemic injury, hypertension and myocarditis.
心脏巨噬细胞维持着内环境稳态，并协调对疾病的反应。遗传命运图谱和单细胞 RNA 测序的应用改变了巨噬细胞异质性的模式，从经典的 M1/M2 分类转向了一种更细致的方法，这种方法调和了不同的起源、生命周期和转录状态。在这里，我们提供了一个概念框架来评估心脏巨噬细胞的复杂性，该框架整合了与亚群特异性标志物（TIMD4 和 CCR2）相关的转录和功能异质性。我们的目标是为研究人员提供一个起点，以剖析已知的驻留心脏巨噬细胞亚群的功能。我们讨论了在缺血性损伤、高血压和心肌炎中我们对心脏巨噬细胞多样性的理解的最新进展和局限性。
