Department of Bioscience and Bioengineering, Indian Institute of Technology Guwahai, Guwahati, 781039, India.
Department of Botany, Bioinformatics and Climate Change, Gujarat University, Navrangpura, Ahmedabad, 380009, India.
Plant Physiol Biochem. 2020 Dec;157:229-238. doi: 10.1016/j.plaphy.2020.10.021. Epub 2020 Oct 27.
Mungbean, is a widely cultivated pulse crop in India, experiences severe drought stress during the cultivation period. The mechanism of drought tolerance in mungbean is not well understood. In this presents study we screened 7 widely cultivated mungbean genotypes towards their drought sensitivity at seedling stage and transcriptome sequencing of drought-tolerant and susceptible genotype to understand the drought tolerance mechanism. Our physiological data such as increase in root length, shoot length, fresh weight, dry weight, relative water content (RWC), proline content, MDA content and molecular data in terms of quantitative expression of drought stress responsive genes under 3-d drought stress in mungbean suggests that, K851 seems to be most drought tolerant and PDM-139 as drought susceptible genotype. The transcriptomic study between K-851 and PDM-139 revealed 22,882 differentially expressed genes (DEGs) which were identified under drought stress, and they were mainly mapped to phytohormone signal transduction, carbon metabolism and flavonoid biosynthesis. Out of these, 10,235 genes were up-regulated and 12,647 genes were down-regulated. Furthermore, we found that, the DEGs related to, phytohormone signal transduction, carbon metabolism and flavonoid biosynthesis and they were more induced in K-851. Our data suggested that, the drought tolerant genotype K-851, scavenges the damage of drought stress by producing more amount of osmolytes, ROS scavengers and sugar biosynthesis.
绿豆在印度是一种广泛种植的豆类作物，在种植期间会经历严重的干旱胁迫。绿豆的耐旱机制尚不清楚。在本研究中，我们对 7 个广泛种植的绿豆品种进行了筛选，以了解其在幼苗期对干旱的敏感性，并对耐旱和敏感基因型进行了转录组测序，以了解耐旱机制。我们的生理数据，如根长、茎长、鲜重、干重、相对含水量 (RWC)、脯氨酸含量、MDA 含量以及绿豆在 3 天干旱胁迫下干旱胁迫响应基因的定量表达的分子数据表明，K851 似乎是最耐旱的，而 PDM-139 则是耐旱的。K-851 和 PDM-139 之间的转录组研究揭示了 22882 个在干旱胁迫下差异表达的基因（DEGs），它们主要被映射到植物激素信号转导、碳代谢和类黄酮生物合成。其中，有 10235 个基因上调，12647 个基因下调。此外，我们发现与植物激素信号转导、碳代谢和类黄酮生物合成相关的 DEGs，在 K-851 中诱导表达更高。我们的数据表明，耐旱基因型 K-851 通过产生更多的渗透物、ROS 清除剂和糖生物合成来清除干旱胁迫的损伤。
