Diaz I, Pedersen S, Kurland C G
Department of Molecular Biology, Biomedical Center, Uppsala, Sweden.
Mol Gen Genet. 1987 Jul;208(3):373-6. doi: 10.1007/BF00328126.
We have measured the growth rates and elongation rates for different proteins in wild-type, miaA, rpsL, and miaA, rpsL double mutants of Escherichia coli in the presence as well as the absence of streptomycin. The data show that while miaA and rpsL mutants inhibit elongation rates to equivalent levels, miaA inhibits the growth rate twice as effectively as does rpsL. The double mutant is more effectively inhibited than either single mutant and Sm repairs in part the growth rate as well as protein elongation rates. The data suggest that the conditional streptomycin-dependent phenotype of the double mutant cannot be due simply to the depressed polypeptide elongation rates of the double mutant.
我们已经测定了在有链霉素和无链霉素存在的情况下，野生型、miaA、rpsL以及miaA、rpsL双突变大肠杆菌中不同蛋白质的生长速率和延伸速率。数据表明，虽然miaA和rpsL突变体将延伸速率抑制到相同水平，但miaA对生长速率的抑制效果是rpsL的两倍。双突变体比任何一个单突变体都更有效地受到抑制，并且链霉素部分恢复了生长速率以及蛋白质延伸速率。数据表明，双突变体的条件性链霉素依赖性表型不能简单地归因于双突变体中多肽延伸速率的降低。
