Department of Medicine and Epidemiology, University of California Davis, Davis, CA 95616, USA.
Mira Vista Diagnostics, Indianapolis, IN 46214, USA.
Vet J. 2020 Nov;265:105551. doi: 10.1016/j.tvjl.2020.105551. Epub 2020 Sep 25.
A commercial Aspergillus galactomannan antigen (GMA) enzyme linked immunosorbent assay (ELISA) is used to support a diagnosis of systemic aspergillosis in dogs. In human patients, false positive results have been associated with administration of medications derived from molds. We sought to determine the effect of administration of a commercially available oral probiotic nutraceutical that contained Aspergillus-derived ingredients on serum and urine Aspergillus GMA levels in dogs by conducting a prospective, cross-over study. Galactomannan index (GMI) was measured on the solubilized probiotic nutraceutical and was positive (GMI ≥ 0.5) with a mean of 7.91. Serum and urine galactomannan indices were measured in 10 healthy dogs before (day 0) and after 1 week (day 7) of probiotic nutraceutical administration, then again 2 weeks after the probiotic nutraceutical was discontinued (day 21). Median (range) serum GMI were 0.19 (0.08-0.62), 0.22 (0.07-1.15) and 0.17 (0.14-0.63) at day 0, 7 and 21, respectively. Two of 10 dogs developed positive GMI (≥0.5) results after probiotic nutraceutical administration; however, no significant changes were noted over the study period. Median (range) urine GMI results were 0.06 (0.04-0.22), 0.07 (0.05-0.41) and 0.06 (0.03-0.16) at day 0, 7 and 21, respectively. A trend towards an increase urine GMI was noted between day 0 and 7 (P = 0.18), and decrease was noted between day 7 and 21 (P = 0.09). Administration of probiotics containing Aspergillus-derived ingredients to dogs did not reliably result in elevated Aspergillus GMA levels.
一种商业化的曲霉半乳甘露聚糖抗原（GMA）酶联免疫吸附试验（ELISA）用于支持犬系统性曲霉病的诊断。在人类患者中，假阳性结果与使用来源于霉菌的药物有关。我们通过进行前瞻性交叉研究，旨在确定给予含有曲霉衍生成分的市售口服益生菌营养补充剂对犬血清和尿液曲霉 GMA 水平的影响。测定了溶解的益生菌营养补充剂的半乳甘露聚糖指数（GMI），并发现其呈阳性（GMI≥0.5），平均值为 7.91。10 只健康犬在给予益生菌营养补充剂前（第 0 天）和 1 周后（第 7 天）以及停止给予益生菌营养补充剂 2 周后（第 21 天）测量血清和尿液半乳甘露聚糖指数。第 0、7 和 21 天的中位数（范围）血清 GMI 分别为 0.19（0.08-0.62）、0.22（0.07-1.15）和 0.17（0.14-0.63）。在给予益生菌营养补充剂后，有 2 只犬的 GMI（≥0.5）呈阳性结果；然而，在研究期间未观察到显著变化。第 0、7 和 21 天的中位数（范围）尿液 GMI 结果分别为 0.06（0.04-0.22）、0.07（0.05-0.41）和 0.06（0.03-0.16）。在第 0 天和第 7 天之间，尿液 GMI 呈增加趋势（P=0.18），在第 7 天和第 21 天之间，尿液 GMI 呈下降趋势（P=0.09）。给予含有曲霉衍生成分的益生菌并未可靠地导致曲霉 GMA 水平升高。
