单链核酸内切酶从膜复合物中释放大肠杆菌DNA。
Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases.
作者信息
Abe M, Brown C, Hendrickson W G, Boyd D H, Clifford P, Cote R H, Schaechter M
出版信息
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2756-60. doi: 10.1073/pnas.74.7.2756.
Abstract
Treatment of gently prepared lysates of Escherichia coli with single-strand-specific endonuclease (SI or from mung beans) results in the release of about 90% of the DNA from membranes, as determined by the M band technique. The released DNA has an average molecular weight of about 1.2 X 10(8). Data obtained with endonuclease S1 fit a mathematical model in which substrate sites are at or near membrane attachment sites. Data obtained with pancreatic deoxyribonuclease or x-rays fit a model for double-strand breaks at random sites along the DNA. Fitting data to these models, we estimate that there are 18+/-5 membrane attachment sites. The DNA remaining after S1 nuclease treatment is enriched for the region near the origin of chromosome replication. Therefore, attachment at this region near the origin of chromosome replication. Therefore, attachment at this region appears to be chemically different from that at the other sites along the DNA.
摘要
用单链特异性核酸内切酶（S1或绿豆核酸酶）处理经温和制备的大肠杆菌裂解物，通过M带技术测定，约90%的DNA从膜上释放出来。释放出的DNA平均分子量约为1.2×10⁸ 。用核酸酶S1获得的数据符合一个数学模型，其中底物位点位于膜附着位点处或其附近。用胰腺脱氧核糖核酸酶或X射线获得的数据符合一个关于DNA上随机位点双链断裂的模型。将数据拟合到这些模型中，我们估计有18±5个膜附着位点。S1核酸酶处理后剩余的DNA在染色体复制起点附近的区域富集。因此，在染色体复制起点附近的这个区域的附着似乎在化学性质上与DNA上其他位点的附着不同。
相似文献
1
Release of Escherichia coli DNA from membrane complexes by single-strand endonucleases.单链核酸内切酶从膜复合物中释放大肠杆菌DNA。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2756-60. doi: 10.1073/pnas.74.7.2756.
2
S1-sensitive sites in DNA after gamma-irradiation.γ射线照射后DNA中的S1敏感位点。
Biochim Biophys Acta. 1981 Feb 26;652(2):261-5. doi: 10.1016/0005-2787(81)90115-5.
3
Effect of ribonuclease on the association of deoxyribonucleic acid with the membrane in Escherichia coli.核糖核酸酶对大肠杆菌中脱氧核糖核酸与细胞膜结合的影响。
J Bacteriol. 1975 May;122(2):592-8. doi: 10.1128/jb.122.2.592-598.1975.
4
Action of gamma endonuclease on clustered lesions in irradiated DNA.γ核酸内切酶对辐照DNA中簇状损伤的作用。
Radiat Environ Biophys. 1988;27(2):123-32. doi: 10.1007/BF01214602.
5
Processing of model single-strand breaks in phi X-174 RF transfecting DNA by Escherichia coli.大肠杆菌对φX - 174 RF转染DNA中模型单链断裂的处理。
Radiat Res. 1991 Jun;126(3):357-66.
6
Specificity of the S1 nuclease from Aspergillus oryzae.米曲霉S1核酸酶的特异性
J Biol Chem. 1975 Nov 25;250(22):8848-55.
7
S1 nuclease hydrolysis of single-stranded nucleic acids with partial double-stranded configuration.S1核酸酶对具有部分双链结构的单链核酸的水解作用。
Biochemistry. 1975 Sep 23;14(19):4221-6. doi: 10.1021/bi00690a011.
8
A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases.一种对单链特异性核酸内切酶高度敏感的鸡重复DNA序列。
Nucleic Acids Res. 1983 Dec 10;11(23):8495-508. doi: 10.1093/nar/11.23.8495.
9
Effect of growth conditions on DNA-membrane attachment in Escherichia coli.生长条件对大肠杆菌中DNA与膜附着的影响。
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2446-50. doi: 10.1073/pnas.72.6.2446.
10
Purification and properties of S1 nuclease from Aspergillus.来自曲霉的S1核酸酶的纯化及性质
Methods Enzymol. 1980;65(1):248-55. doi: 10.1016/s0076-6879(80)65034-4.
引用本文的文献
1
The Birth of the Copenhagen School: Personal Recollections at the EMBO Workshop on Bacterial Growth Physiology, 2022.哥本哈根学派的诞生：2022年欧洲分子生物学组织细菌生长生理学研讨会上的个人回忆
Life (Basel). 2023 Nov 21;13(12):2235. doi: 10.3390/life13122235.
2
Enrichment of DNA polymerase III activity in a DNA membrane complex purified from Pneumococcus: the possible existence of subcomplexes.从肺炎球菌中纯化的DNA膜复合物中DNA聚合酶III活性的富集：亚复合物可能的存在。
Mol Gen Genet. 1981;182(1):87-94. doi: 10.1007/BF00422772.
3
Membrane-DNA attachment sites in Streptococcus faecalis cells grown at different rates.不同生长速率下粪肠球菌细胞中的膜-DNA附着位点。
J Bacteriol. 1982 Oct;152(1):191-200. doi: 10.1128/jb.152.1.191-200.1982.
4
Recognition sites for a membrane-derived DNA binding protein preparation in the E. coli replication origin.大肠杆菌复制起点中一种膜衍生DNA结合蛋白制剂的识别位点。
Mol Gen Genet. 1983;191(3):460-5. doi: 10.1007/BF00425763.
5
Identification of specific restriction fragments associated with a membrane subparticle from Bacillus subtilis.与枯草芽孢杆菌膜亚颗粒相关的特定限制性片段的鉴定。
J Bacteriol. 1983 Jun;154(3):1389-96. doi: 10.1128/jb.154.3.1389-1396.1983.
6
Isolation of a replication origin complex from Escherichia coli.从大肠杆菌中分离复制起始复合物。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):262-6. doi: 10.1073/pnas.77.1.262.
7
Membrane particles from Escherichia coli and Bacillus subtilis, containing penicillin-binding proteins and enriched for chromosomal-origin DNA.来自大肠杆菌和枯草芽孢杆菌的膜颗粒，含有青霉素结合蛋白并富含染色体起源的DNA。
J Bacteriol. 1985 Oct;164(1):192-200. doi: 10.1128/jb.164.1.192-200.1985.
8
Identification of a specific membrane-particle-associated DNA sequence in Bacillus subtilis.枯草芽孢杆菌中一种特定的膜颗粒相关DNA序列的鉴定。
J Bacteriol. 1986 Apr;166(1):38-43. doi: 10.1128/jb.166.1.38-43.1986.
9
Positioning of replicated chromosomes in Escherichia coli.大肠杆菌中复制染色体的定位
J Bacteriol. 1990 Jan;172(1):31-9. doi: 10.1128/jb.172.1.31-39.1990.
10
Evidence for the specific association of the chromosomal origin with outer membrane fractions isolated from Escherichia coli.染色体起源与从大肠杆菌中分离出的外膜组分之间存在特定关联的证据。
J Bacteriol. 1978 Jul;135(1):178-89. doi: 10.1128/jb.135.1.178-189.1978.
本文引用的文献
1
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.大肠杆菌噬菌体及大肠杆菌噬菌体DNA的分子量。IV. 来自噬菌体T4、T5和T7的DNA的分子量以及分子量测定的一般问题
J Mol Biol. 1970 Dec 28;54(3):567-77. doi: 10.1016/0022-2836(70)90127-0.
2
Shear sensitivity of the E. coli genome: multiple membrane attachment points of the E. coli DNA.大肠杆菌基因组的剪切敏感性：大肠杆菌DNA的多个膜附着点。
Cold Spring Harb Symp Quant Biol. 1968;33:65-72. doi: 10.1101/sqb.1968.033.01.012.
3
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.米曲霉单链特异性核酸酶的纯化及其他特性
Eur J Biochem. 1973 Feb 15;33(1):192-200. doi: 10.1111/j.1432-1033.1973.tb02669.x.
4
Structural uniqueness of lactose operator.乳糖操纵子的结构独特性。
Nature. 1974 Nov 15;252(5480):205-9. doi: 10.1038/252205a0.
5
Effect of rifampin on the structure and membrane attachment of the nucleoid of Escherichia coli.利福平对大肠杆菌类核结构及膜附着的影响。
J Bacteriol. 1973 Dec;116(3):1364-74. doi: 10.1128/jb.116.3.1364-1374.1973.
6
On the structure of the folded chromosome of Escherichia coli.关于大肠杆菌折叠染色体的结构
J Mol Biol. 1972 Nov 14;71(2):127-47. doi: 10.1016/0022-2836(72)90342-7.
7
Sites of replication of chromosomal DNA in a eukaryotic cell.真核细胞中染色体DNA的复制位点。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2300-5. doi: 10.1073/pnas.69.8.2300.
8
Interactions between twisted DNAs and enzymes: the effects of superhelical turns.扭曲DNA与酶之间的相互作用：超螺旋圈数的影响。
J Mol Biol. 1974 Aug 25;87(4):797-816. doi: 10.1016/0022-2836(74)90085-0.
9
The attachment of the bacterial chromosome to the cell membrane.
Int Rev Cytol. 1975;41:1-28. doi: 10.1016/s0074-7696(08)60964-x.
10
S1 nuclease converts relaxed circular closed duplex DNA to open linear DNA of unit length.S1核酸酶可将松弛的环状闭合双链DNA转化为单位长度的开放线性DNA。
FEBS Lett. 1976 Sep 15;68(1):68-70. doi: 10.1016/0014-5793(76)80406-1.
Zotero 插件