（爬行动物：巨蜥科）消化道内分泌细胞的免疫组织化学研究
An immunohistochemical study of endocrine cells in the digestive tract of (Reptile: Varanidae).
作者信息
Mahfud Mahfud, Ernawati Ernawati, Mahmud Nur R Adawiyah, Budipitojo Teguh, Wijayanto Hery
机构信息
Department of Biology Education, University of Muhammadiyah Kupang, East of Nusa Tenggara Province, Indonesia.
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Gajah Mada University, Yogyakarta, Indonesia.
出版信息
Vet World. 2020 Sep;13(9):1737-1742. doi: 10.14202/vetworld.2020.1737-1742. Epub 2020 Sep 1.
AIM
The aim of the study was to identify the distribution pattern and frequency of endocrine cell types in the digestive tract of .
MATERIALS AND METHODS
The presence of endocrine cells (glucagon, somatostatin, and serotonin) in the digestive tract (esophagus, stomach, and intestine) was detected using the avidin-biotin complex (ABC) method.
RESULTS
Three types of endocrine cells immunoreactive to antisera glucagon, serotonin, and somatostatin were found in the caudal portion of the small and large intestines but were not observed in the esophagus, stomach, and caput and medial sections of the small intestine. Endocrine cells distributed in the digestive tract of vary in color intensity, from weak to sharp, in response to the primer antibody.
CONCLUSION
Endocrine cells in the digestive tract that is immunoreactive to glucagon, somatostatin, and serotonin are those found in the caudal portion of the small and large intestines. They are varied in distribution pattern, frequency, and color intensity.
目的
本研究的目的是确定[具体对象]消化道内分泌细胞类型的分布模式和频率。
材料与方法
采用抗生物素蛋白-生物素复合物（ABC）法检测消化道（食管、胃和肠）中内分泌细胞（胰高血糖素、生长抑素和5-羟色胺）的存在情况。
结果
在小肠和大肠尾部发现了三种对胰高血糖素、5-羟色胺和生长抑素抗血清呈免疫反应的内分泌细胞，但在食管、胃以及小肠头部和中部未观察到。[具体对象]消化道中分布的内分泌细胞，根据一抗的不同，颜色强度从弱到强不等。
结论
对胰高血糖素、生长抑素和5-羟色胺呈免疫反应的消化道内分泌细胞存在于小肠和大肠尾部。它们在分布模式、频率和颜色强度上各不相同。
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