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从家禽和人类中分离出的 spp. 的抗菌药物耐药性分子特征分析以及肠杆菌重复基因间共识聚合酶链反应作为一种分子分型工具

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for spp. isolated from poultry and humans.

作者信息

Herrera-Sánchez María Paula, Rodríguez-Hernández Roy, Rondón-Barragán Iang Schroniltgen

机构信息

Research Group in Immunology and Pathogenesis, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué, Tolima, Colombia.

Poultry Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Santa Helena Highs, Ibagué, Tolima, Colombia.

出版信息

Vet World. 2020 Sep;13(9):1771-1779. doi: 10.14202/vetworld.2020.1771-1779. Epub 2020 Sep 4.

Abstract

BACKGROUND AND AIM

spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success. This study was conducted to characterize the phenotypic and genotypic antibiotic resistance profile of strains isolated from broilers and humans from the regions of Tolima and Santander (Colombia).

MATERIALS AND METHODS

spp. strains (n=49) were confirmed through molecular detection by amplification of the gene. Phenotypic antibiotic resistance was determined by the automated method and the agar diffusion method, and the presence of resistance genes was evaluated by PCR. Genotypic characterization was conducted using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, from which a dendrogram was generated and the possible phylogenetic relationships were established.

RESULTS

isolates were classified as MDR strains exhibiting resistance to four antibiotic classes, penicillins, aminoglycosides, sulfonamides, and cephalosporins, and the human strains were resistant to gentamicin. At the genotypic level, the isolates contained the genes , , , , , , , , and . The genotyping results obtained by ERIC-PCR allowed the grouping of strains according to the source of isolation.

CONCLUSION

The spp. strains exhibited resistance to multiple antibiotics, as well as multiple genes associated with them, and the ERIC-PCR method was a technique that was helpful in generating clusters with biological significance.

摘要

背景与目的

沙门氏菌是世界上最重要的食源性病原体之一,已成为主要的公共卫生问题。此外,已从沙门氏菌病暴发中分离出多重耐药(MDR)菌株,这影响了其治疗效果。本研究旨在对从托利马省和桑坦德省(哥伦比亚)的肉鸡和人类中分离出的沙门氏菌菌株的表型和基因型抗生素耐药谱进行表征。

材料与方法

通过对invA基因进行扩增的分子检测来确认49株沙门氏菌菌株。采用自动化方法和琼脂扩散法测定表型抗生素耐药性,并通过PCR评估耐药基因的存在情况。使用肠杆菌重复基因间共识(ERIC)-PCR方法进行基因型表征,由此生成树状图并建立可能的系统发育关系。

结果

分离株被分类为对四类抗生素(青霉素类、氨基糖苷类、磺胺类和头孢菌素类)耐药的多重耐药菌株,且人类菌株对庆大霉素耐药。在基因型水平上,分离株含有blaTEM、blaSHV、blaCTX-M、tetA、tetB、tetC、tetD、tetG、sul1和sul2基因。通过ERIC-PCR获得的基因分型结果能够根据分离来源对菌株进行分组。

结论

沙门氏菌菌株表现出对多种抗生素以及与之相关的多个基因的耐药性,并且ERIC-PCR方法是一种有助于生成具有生物学意义的聚类的技术。

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