Torres-Elizalde Lilibeth, Ortega-Paredes David, Loaiza Karen, Fernández-Moreira Esteban, Larrea-Álvarez Marco
School of Biological Science and Engineering, Yachay-Tech University, Hacienda San José, Urcuquí 100650, Ecuador.
Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR), Facultad de Medicina Veterinaria y Zootecnia, Universidad Central del Ecuador, Quito 170129, Ecuador.
Antibiotics (Basel). 2021 Nov 12;10(11):1388. doi: 10.3390/antibiotics10111388.
Antimicrobial resistance genes are often associated with integrons, which promote their movement between and within DNA molecules. IntFinder 1.0 and I-VIP v1.2 were used for the detection of integrons and their associated resistance genes in assembled sequences and raw reads. A dataset comprising 1688 sequenced isolates from countries of the Andean Community was developed. A total of 749 and 680 integrons were identified by IntFinder 1.0 and I-VIP v1.2, respectively; class 2 integrons were the most abundant followed by class 1, whereas no class 3 integrons were detected. These elements were mainly associated with isolates from animal sources. Infantis ST32 contained the majority of integrons. Trimethoprim resistance genes () were found in greater numbers than others, including and genes. The presence of these resistance integrons may come as a response to antibiotic misuse, especially of co-trimoxazole. This represents a public health risk as novel resistant strains might appear due to gene dissemination. The information gathered from in silico studies not only contributes to our understanding of integron dynamics in pathogenic , but also helps identify potential emergent patterns of resistance in the region, which is fundamental for developing pertinent antibiotic surveillance programs.
抗菌耐药基因通常与整合子相关联，整合子促进它们在DNA分子之间以及分子内部移动。IntFinder 1.0和I-VIP v1.2用于在组装序列和原始读数中检测整合子及其相关的耐药基因。构建了一个包含来自安第斯共同体国家的1688个测序分离株的数据集。IntFinder 1.0和I-VIP v1.2分别鉴定出749个和680个整合子；2类整合子最为丰富，其次是1类，未检测到3类整合子。这些元件主要与动物源分离株相关。婴儿ST32含有大多数整合子。甲氧苄啶耐药基因（）的数量比其他基因更多，包括和基因。这些耐药整合子的存在可能是对抗生素滥用的一种反应，尤其是对复方新诺明的滥用。这代表了一种公共卫生风险，因为基因传播可能会出现新的耐药菌株。从计算机模拟研究中收集的信息不仅有助于我们了解致病中的整合子动态，还有助于识别该地区潜在的新出现的耐药模式，这对于制定相关的抗生素监测计划至关重要。
