Rahayuningtyas Irma, Indrawati Agustin, Wibawan I Wayan Teguh, Palupi Maria Fatima, Istiyaningsih Istiyaningsih
Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University-Bogor, Indonesia.
National Veterinary Drug Assay Laboratory, Directorate General of Livestock and Animal Health, Ministry of Agriculture of the Republic of Indonesia, Indonesia.
Vet World. 2020 Sep;13(9):1807-1814. doi: 10.14202/vetworld.2020.1807-1814. Epub 2020 Sep 5.
Pathogenic contamination along the broiler meat supply chain is a serious public health concern. This bacterial infection with multidrug-resistant can lead to treatment failure. Several studies have revealed that avian pathogenic (APEC) and human extraintestinal pathogenic (ExPEC) showed a close genetic relationship and may share virulence genes. This study aimed to determine the phylogenetic group and virulence gene profiles in colistin-resistant obtained from the broiler meat supply chain in Bogor, West Java, Indonesia.
Fifty-eight archive isolates originated from the cloacal swab, litter, drinking water, inside plucker swab, fresh meat at small scale poultry slaughterhouses, and traditional markets were used in this study. All the isolates were characterized by a polymerase chain reaction to determine the phylogenetic group (A, B1, B2, or D) and virulence gene profiles with APEC marker genes (, , , , and ).
Phylogenetic grouping revealed that the isolates belong to A group (34.48%), D group (34.48%), B1 group (17.24%), and B2 group (13.79%). The virulence gene prevalence was as follows: (36%), (21%), (21%), (10%), and (9%). The B2 group presented with more virulence genes combinations. , , and genes were positively associated with the B2 group (p≤0.05).
Our results highlight the role of colistin-resistant originated from the broiler meat supply chain as a potential reservoir for human ExPEC virulence genes.
肉鸡供应链中的致病性污染是一个严重的公共卫生问题。这种具有多重耐药性的细菌感染可能导致治疗失败。多项研究表明，禽致病性大肠杆菌（APEC）和人类肠道外致病性大肠杆菌（ExPEC）显示出密切的遗传关系，并且可能共享毒力基因。本研究旨在确定从印度尼西亚西爪哇茂物的肉鸡供应链中获得的耐黏菌素大肠杆菌的系统发育群和毒力基因谱。
本研究使用了58株存档菌株，这些菌株分别来源于泄殖腔拭子、垫料、饮用水、拔毛机内部拭子、小型家禽屠宰场的鲜肉以及传统市场。所有菌株通过聚合酶链反应进行特征分析，以确定系统发育群（A、B1、B2或D）以及带有APEC标记基因（、、、和）的毒力基因谱。
系统发育分组显示，这些菌株属于A组（34.48%）、D组（34.48%）、B1组（17.24%）和B2组（13.79%）。毒力基因的流行情况如下：（36%）、（21%）、（21%）、（10%）和（9%）。B2组呈现出更多的毒力基因组合。、和基因与B2组呈正相关（p≤0.05）。
我们的结果突出了源自肉鸡供应链的耐黏菌素大肠杆菌作为人类ExPEC毒力基因潜在储存库的作用。
