Department of Veterinary Medicine, College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
College of Traditional Chinese Medicine, Zhanjiang University of Science and Technology, Zhanjiang, China.
Front Cell Infect Microbiol. 2023 Jun 15;13:1202013. doi: 10.3389/fcimb.2023.1202013. eCollection 2023.
The widespread use of antibiotics in animal agriculture has increased the resistance of , and pathogenic often harbor complex virulence factors. Antimicrobial resistance in pathogenic bacteria can cause public health problems. Correlation analyses of the resistance, virulence, and serotype data from the pathogenic bacteria found on farms and in the surrounding environment can thus provide extremely valuable data to help improve public health management.
In this investigation, we have assessed the drug resistance and virulence genes as well as the molecular typing characteristics of 30 strains isolated from duck farms in the Zhanjiang area of China. Polymerase chain reaction was used to detect the drug resistance and virulence genes as well as serotypes, and whole-genome sequencing was used to analyze the multilocus sequence typing.
The detection rates for the resistance gene and virulence gene were highest (93.3%, respectively). There were no correlations between the drug resistance and virulence gene numbers in the same strain. The epidemic serotype was O81 (5/24), ST3856 was an epidemic sequence type, and strains I-9 and III-6 carried 11 virulence genes. The strains from the duck farms in the Zhanjiang area were thus found to have a broad drug resistance spectrum, various virulence genes, complex serotypes, and certain pathogenicity and genetic relationship.
Monitoring the spread of pathogenic bacteria and the provision of guidance regarding the use of antibiotics in the livestock and poultry industries will be required in the future in the Zhanjiang area.
在动物农业中广泛使用抗生素增加了病原菌的耐药性，而且病原菌通常具有复杂的毒力因子。病原菌的抗生素耐药性会引发公共卫生问题。因此，对农场和周围环境中分离的病原菌的耐药性、毒力和血清型数据进行相关性分析，可以提供非常有价值的数据，有助于改善公共卫生管理。
在本研究中，我们评估了来自中国湛江地区养鸭场的 30 株分离株的耐药性和毒力基因以及分子分型特征。聚合酶链反应用于检测耐药性和毒力基因以及血清型，全基因组测序用于分析多位点序列分型。
耐药基因和毒力基因的检出率最高（分别为 93.3%）。同一菌株中耐药基因和毒力基因数量之间没有相关性。流行血清型为 O81（24 株中的 5 株），流行序列型为 ST3856，菌株 I-9 和 III-6 携带 11 个毒力基因。因此，湛江地区养鸭场的病原菌具有广泛的耐药谱、多种毒力基因、复杂的血清型以及一定的致病性和遗传关系。
未来湛江地区需要监测病原菌的传播，并为畜牧业和家禽业抗生素的使用提供指导。
