介导有效消除多重耐药性禽致病性细菌的噬菌体
Bacteriophages Mediating Effective Elimination of Multidrug-Resistant Avian Pathogenic .
作者信息
Koratkar Santosh, Bhutada Pankhudi, Giram Pranoti, Verma Chetan, Saroj Sunil D
机构信息
Symbiosis School of Biological Sciences, Symbiosis International [Deemed University], Lavale, India.
出版信息
Phage (New Rochelle). 2024 Jun 21;5(2):76-83. doi: 10.1089/phage.2023.0018. eCollection 2024 Jun.
BACKGROUND
Avian pathogenic (APEC) causes colibacillosis and septicemia; in certain cases, mortality leads to economic losses and elicits potential foodborne zoonotic risk. The study aimed to determine the prevalence of APEC pathotypes and serotypes in poultry, followed by characterization for virulence markers and antibiotic sensitivity and analysis of lytic efficacy of bacteriophages in the eradication of APEC.
METHODS
We successfully isolated and characterized 34 isolates from poultry farms. The lytic efficacy of seven bacteriophages, as well as a phage cocktail, was evaluated for biological control of multiple drug resistance (MDR) APEC.
RESULTS
A total of 67.65% of isolated were APEC. A total of 94.11% of the isolates were multidrug-resistant bacteria harboring virulence genes. The lytic ability of seven bacteriophages ranged from 0.98% to 36.76%, with a cocktail of EscoΦA-06 and ΦA-07 exhibiting lysis of 48.04% isolates.
CONCLUSION
As serological variability in APEC limits the application and development of vaccines, the findings support the employment of bacteriophages against elimination of MDR APEC in poultry settings.
背景
禽致病性大肠杆菌（APEC）可引起大肠杆菌病和败血症；在某些情况下，死亡率会导致经济损失，并引发潜在的食源性人畜共患病风险。本研究旨在确定家禽中APEC致病型和血清型的流行情况，随后对毒力标记物和抗生素敏感性进行鉴定，并分析噬菌体在根除APEC中的裂解效果。
方法
我们成功地从家禽养殖场分离并鉴定了34株菌株。评估了七种噬菌体以及一种噬菌体鸡尾酒对多重耐药（MDR）APEC的生物防治裂解效果。
结果
总共67.65%的分离株为APEC。总共94.11%的分离株是携带毒力基因的多重耐药菌。七种噬菌体的裂解能力在0.98%至36.76%之间，EscoΦA-06和ΦA-07的混合噬菌体对48.04%的分离株有裂解作用。
结论
由于APEC中的血清学变异性限制了疫苗的应用和开发，这些发现支持在家禽环境中使用噬菌体来消除MDR APEC。
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