对应于大肠杆菌中五个趋化性基因的核苷酸序列。
Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli.
作者信息
Mutoh N, Simon M I
出版信息
J Bacteriol. 1986 Jan;165(1):161-6. doi: 10.1128/jb.165.1.161-166.1986.
Abstract
The nucleotide sequence of DNA which contains five chemotaxis-related genes of Escherichia coli, cheW, cheR, cheB, cheY, and cheZ, and part of the cheA gene was determined. Molecular weights of the polypeptides encoded by these genes were calculated from translated amino acid sequences, and they were 18,100 for cheW, 32,700 for cheR, 37,500 for cheB, 14,100 for cheY, and 24,000 for cheZ. Nucleotide sequences which could act as ribosome-binding sites were found in the upstream region of each gene. After the termination codon of the cheW gene, a typical rho-independent transcription termination signal was observed. There are no other open reading frames long enough to encode polypeptides in this region except those which code for the two previously reported genes tar and tap.
摘要
测定了包含大肠杆菌五个趋化相关基因（cheW、cheR、cheB、cheY和cheZ）以及部分cheA基因的DNA核苷酸序列。根据翻译后的氨基酸序列计算了这些基因编码的多肽的分子量，cheW为18,100，cheR为32,700，cheB为37,500，cheY为14,100，cheZ为24,000。在每个基因的上游区域发现了可作为核糖体结合位点的核苷酸序列。在cheW基因的终止密码子之后，观察到一个典型的不依赖ρ因子的转录终止信号。除了编码两个先前报道的基因tar和tap的那些序列外，该区域没有其他足够长的开放阅读框来编码多肽。
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