Savić Sara, Stosic Marina Zekic, Marcic Doroteja, Hernández Isabel, Potkonjak Aleksandar, Otasevic Suzana, Ruzic Maja, Morchón Rodrigo
Scientific Veterinary Institute "Novi Sad", Novi Sad, Serbia.
Group of Animal and Human Dirofilariasis, Faculty of Pharmacy, Campus Miguel Unamuno, University of Salamanca, Salamanca, Spain.
Front Vet Sci. 2020 Sep 29;7:571. doi: 10.3389/fvets.2020.00571. eCollection 2020.
Dirofilariasis is a vector-borne zoonotic disease caused mainly by and that affect dogs and humans all over the world. Serbia is considered an endemic country to both forms of dirofilariasis, although most of the population is concentrated in the north of the country. The aims of this study were to show the prevalence of and in dogs and the seroprevalence in humans compared to previous studies in Northern Serbia. In total, 346 dog sera samples and 265 human samples were analyzed. Dog blood samples were analyzed using the modified Knott's method to check whether there were spp. microfilariae and serum samples were checked by a commercial antigen test. Human serum samples were analyzed with a non-commercial ELISA for detection of specific anti-, anti-, and anti- IgG antibodies, and confirmed by western blotting. The overall prevalence for spp. in dogs was 29.19%. The overall prevalence for was 26.30%. The percentages of and microfilaremia in dogs were 25.72 and 1.45%, respectively, while ./ microfilaremia co-infections were also 1.45%. The overall seroprevalence for spp. in humans was 3.77%. The overall seroprevalence for was 1.51, 1.13% for , and for / co-infections was 1.13%. The results indicate that and are present in dogs and humans in the province of Vojvodina, in the northern part of Serbia. It is most likely associated with the presence of many rivers, the climate, and presence of mosquitoes in the area, so there could be a real public health risk.
犬恶丝虫病是一种主要由[具体虫名1]和[具体虫名2]引起的媒介传播人畜共患病，影响着全球的犬类和人类。塞尔维亚被认为是两种形式犬恶丝虫病的流行国家，尽管该国大部分人口集中在北部。本研究的目的是与塞尔维亚北部以前的研究相比，展示犬类中[具体虫名1]和[具体虫名2]的流行率以及人类中的血清流行率。总共分析了346份犬血清样本和265份人类样本。犬血样本采用改良的诺氏法进行分析，以检查是否存在[具体虫名1]属微丝蚴，血清样本通过商业[具体抗原名称]抗原检测进行检查。人类血清样本采用非商业ELISA检测特异性抗[具体虫名1]、抗[具体虫名2]和抗[具体虫名1/2] IgG抗体，并通过蛋白质印迹法进行确认。犬类中[具体虫名1]属的总体流行率为29.19%。[具体虫名2]的总体流行率为26.30%。犬类中[具体虫名1]和[具体虫名2]微丝蚴血症的百分比分别为25.72%和1.45%，而[具体虫名1/2]微丝蚴血症合并感染率也为1.45%。人类中[具体虫名1]属的总体血清流行率为3.77%。[具体虫名2]的总体血清流行率为1.51%，[具体虫名3]为1.13%，[具体虫名1/2]合并感染为1.13%。结果表明，在塞尔维亚北部的伏伊伏丁那省，犬类和人类中存在[具体虫名1]和[具体虫名2]。这很可能与该地区众多河流的存在、气候以及蚊子的存在有关，因此可能存在真正的公共卫生风险。
