Maeda Kotaro, Katsuno Hidetoshi, Kono Toru
International Medical Center, Fujita Health University Hospital, Toyoake, Japan.
Department of Surgery, Fujita Health University Okazaki Medical Center, Okazaki, Japan.
J Anus Rectum Colon. 2020 Oct 29;4(4):193-200. doi: 10.23922/jarc.2020-041. eCollection 2020.
This study aimed to elucidate the effect of the extract of daikenchuto (DKT), a Japanese Kampo medicine, on the contractile activity of the internal anal sphincter in conscious dogs.
Force transducers were attached to the serosal surface of the rectum and the internal anal sphincter of male beagle dogs. In addition, the contractile activity of the rectum and the internal anal sphincter was continuously measured until 6 h after DKT administration telemetry in the conscious state. The DKT dose was 1.5 g/body, and the administration route was intrarectal in the expectation of a direct effect on the rectoanal region. DKT was re-administered to the same animal after drug withdrawal, and the plasma concentrations of hydroxy-α-sanshool (HAS) and hydroxy-β-sanshool (HBS) before and after administration were measured.
After DKT administration, the contractile activity of the internal anal sphincter immediately increased, peaked at 10 min, continued for ≥1 h, and had almost disappeared after 4 h. Rectal contraction differed from that of the internal anal sphincter, with no significant contraction observed. HAS and HBS were found in the plasma of animals administered with DKT and persisted up to 2 h after the administration.
This is the first report on telemetry demonstrating that DKT exhibited contractile effects on the dog's internal anal sphincter. The increased anal pressure and improvement of fecal incontinence symptoms observed in previous clinical studies may have been based on this sphincter contraction.
本研究旨在阐明日本汉方药物大建中汤提取物（DKT）对清醒犬肛门内括约肌收缩活动的影响。
将力传感器附着于雄性比格犬直肠和肛门内括约肌的浆膜表面。此外，在清醒状态下通过遥测技术持续测量直肠和肛门内括约肌的收缩活动，直至给予DKT后6小时。DKT剂量为1.5 g/只，给药途径为直肠给药，以期对直肠肛管区域产生直接作用。在停药后对同一动物再次给予DKT，并测量给药前后血浆中羟基-α-山椒素（HAS）和羟基-β-山椒素（HBS）的浓度。
给予DKT后，肛门内括约肌的收缩活动立即增强，在10分钟时达到峰值，持续≥1小时，4小时后几乎消失。直肠收缩与肛门内括约肌不同，未观察到明显收缩。在给予DKT的动物血浆中发现了HAS和HBS，给药后持续存在长达2小时。
这是关于遥测技术的首篇报道，证明DKT对犬的肛门内括约肌具有收缩作用。先前临床研究中观察到的肛门压力升高和大便失禁症状改善可能基于这种括约肌收缩。
