Department of Clinical Laboratory, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Nephrology Department 2, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
J Cell Mol Med. 2020 Dec;24(24):14306-14315. doi: 10.1111/jcmm.16047. Epub 2020 Nov 1.
Circular RNAs (circRNAs) have been demonstrated to play important roles in cancer progress. However, the roles in hepatocellular carcinoma (HCC) are still unclear. Here, we found has_circRNA_001306 (circ_1306) was up-regulated in HCC tissues and cell lines. Knockdown the expression circ_1306 significantly suppressed HCC cell proliferation and induced the cell apoptosis in vitro and in vivo. Furthermore, we identified circ_1306 could up-regulate the expression of CDK16 by sponging miR-584-5p. The expression of miR-584-5p was decreased, and the expression of CDK16 was increased in HCC tissues and cell lines. Meanwhile, either knockdown of miR-584-5p or overexpression of CDK16 could suppress the HCC cell proliferation. In vivo, overexpression of miR-584-5p or knockdown of circ_1306 could inhibit the expression of CDK16, and suppress tumour growth. Altogether, our findings suggested that circ_1306 could promoter HCC progress by miR-584-5p/CDK16 axis, which provided a novel marker for HCC diagnosis and treatment.
环状 RNA（circRNAs）已被证明在癌症进展中发挥重要作用。然而，其在肝细胞癌（HCC）中的作用尚不清楚。在这里，我们发现环状 RNA_001306（circ_1306）在 HCC 组织和细胞系中上调。敲低 circ_1306 的表达可显著抑制 HCC 细胞的增殖，并在体外和体内诱导细胞凋亡。此外，我们发现 circ_1306 可以通过海绵吸附 miR-584-5p 而上调 CDK16 的表达。miR-584-5p 的表达减少，CDK16 的表达在 HCC 组织和细胞系中增加。同时，敲低 miR-584-5p 或过表达 CDK16 均可抑制 HCC 细胞的增殖。在体内，过表达 miR-584-5p 或敲低 circ_1306 均可抑制 CDK16 的表达，抑制肿瘤生长。总之，我们的研究结果表明，circ_1306 可以通过 miR-584-5p/CDK16 轴促进 HCC 的进展，为 HCC 的诊断和治疗提供了一个新的标志物。
