Department of Traditional Chinese Medicine, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
Department of Traditional Chinese Medicine, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
Steroids. 2021 Jan;165:108740. doi: 10.1016/j.steroids.2020.108740. Epub 2020 Oct 31.
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting women of reproductive age. Cangfu Daotan Decoction (CFDTT) is one of the prescriptions in the stagnation of obesity-type PCOS. Our previous study showed that CFDTT treatment of obese PCOS was correlated with organic anion transporting polypeptides (OATPs).
Here, we studied the effects of CFDTT on obese PCOS and its underlying mechanism. We built an obese PCOS rat model and treated the rats with CFDTT. Then, we detected the serum levels of TCHO, TG, LDL-c, HDL-c, luteinizing hormone (LH), follicle stimulating growth hormone (FSH), testosterone (T), estradiol (E), IL-1β, IL-6, and TNF-α in each group and adopted RT-PCR, western blotting and immunohistochemical staining to investigate the effects of CFDTT treatment on the expression of OATP2B1 and OATP3A1 in ovarian and uterine tissues. In addition, we compared the pregnancy outcomes of each group.
We found that CFDTT decreased the serum levels of TCHO, TG, LDL-c, LH, T, IL-1β, IL-6, and TNF-α and increased the levels of HDL-c, FSH and E in a dose-dependent manner. CFDTT could induce the expression of OATP2B1 and OATP3A1 in ovarian and uterine tissues. Moreover, CFDTT could improve pregnancy outcomes.
These data suggested that the therapeutic mechanism of Cangfu Daotan Decoction on PCOS may be correlated with regulating lipid metabolism, sex hormone secretion and the inflammatory response and increasing OATP2B1 and OATP3A1 expression. Cangfu Daotan Decoction can be developed as a PCOS treatment drug.
多囊卵巢综合征（PCOS）是影响育龄妇女的最常见内分泌疾病之一。苍附导痰汤（CFDTT）是肥胖型 PCOS 瘀滞证的方剂之一。我们之前的研究表明，CFDTT 治疗肥胖型 PCOS 与有机阴离子转运多肽（OATPs）有关。
在这里，我们研究了 CFDTT 对肥胖型 PCOS 的影响及其潜在机制。我们建立了肥胖型 PCOS 大鼠模型，并对大鼠进行 CFDTT 治疗。然后，我们检测了各组大鼠血清中 TCHO、TG、LDL-c、HDL-c、促黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）、雌二醇（E）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平，并采用 RT-PCR、western blot 和免疫组织化学染色法研究 CFDTT 治疗对卵巢和子宫组织中 OATP2B1 和 OATP3A1 表达的影响。此外，我们比较了各组的妊娠结局。
我们发现 CFDTT 呈剂量依赖性降低血清 TCHO、TG、LDL-c、LH、T、IL-1β、IL-6 和 TNF-α水平，增加 HDL-c、FSH 和 E 水平。CFDTT 可诱导卵巢和子宫组织中 OATP2B1 和 OATP3A1 的表达。此外，CFDTT 可改善妊娠结局。
这些数据表明，苍附导痰汤治疗 PCOS 的机制可能与调节脂代谢、性激素分泌和炎症反应以及增加 OATP2B1 和 OATP3A1 表达有关。苍附导痰汤可开发为治疗 PCOS 的药物。
