Zhang Meng, Meng Xiaochen, Zhu Lianqing
Beijing Key Laboratory for Optoelectronic Measurement Technology, Beijing Laboratory of Biomedical Testing Technology and Instruments, Beijing Information Science and Technology University, Beijing 100101, P.R.China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2020 Oct 25;37(5):848-854. doi: 10.7507/1001-5515.201911020.
A high throughput measurement method of human red blood cells (RBCs) deformability combined with optical tweezers technology and the microfluidic chip was proposed to accurately characterize the deformability of RBCs statistically. Firstly, the effective stretching deformation of RBCs was realized by the interaction of photo-trapping force and fluid viscous resistance. Secondly, the characteristic parameters before and after the deformation of the single cell were extracted through the image processing method to obtain the deformation index of area and circumference. Finally, statistical analysis was performed, and the average deformation index parameters (, ) were used to characterize the deformability of RBCs. A high-throughput detection system was built, and the optimal experimental conditions were obtained through a large number of experiments. Three groups of samples with different deformability were used for statistical verification. The results showed that the smallest cell component was 9.71%, and the detection flux of 8-channel structure was about 370 cells/min. High-throughput detection and characterization methods can effectively distinguish different deformed RBCs statistically, which provides a solution for high-throughput deformation analysis of other types of samples.
提出了一种结合光镊技术和微流控芯片的人红细胞（RBCs）变形性高通量测量方法，以从统计学角度准确表征红细胞的变形性。首先，通过光阱力与流体粘性阻力的相互作用实现红细胞的有效拉伸变形。其次，通过图像处理方法提取单细胞变形前后的特征参数，以获得面积和周长的变形指数。最后，进行统计分析，并使用平均变形指数参数（，）来表征红细胞的变形性。构建了高通量检测系统，并通过大量实验获得了最佳实验条件。使用三组具有不同变形性的样品进行统计验证。结果表明，最小细胞成分 为9.71%，8通道结构的检测通量约为370个细胞/分钟。高通量检测和表征方法能够从统计学上有效区分不同变形的红细胞，为其他类型样品的高通量变形分析提供了解决方案。
