Department of Physiology, College of Medicine, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.
Department of Crop Production and Soil Science, Faculty of Agricultural Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo, Nigeria.
J Basic Clin Physiol Pharmacol. 2020 Nov 3;32(3):237-246. doi: 10.1515/jbcpp-2019-0313.
Cardiovascular diseases are major causes of non-infectious diseases globally. The use of pesticides has been linked with the high global burden of non-communicable diseases. Despite the indiscriminate exposure to dichlorvos (DDVP) by inhalation, no report exists on its possible cardiotoxic effect. This study investigated the cardiotoxicity of DDVP exposure by inhalation and the possible role of seed oil.
Twenty-one male rats were randomly assigned into 3 groups. Group A (control) received only standard rat diet and water ad' libitum, group B (DDVP) was exposed to DDVP via inhalation for 15 min daily in addition to rat diet and water, and group C (DDVP + . seed oil) received treatment as group B as well as 300 mg/kg of . seed oil for 28 days.
Significant reductions in body weight gain and cardiac weight were observed in DDVP-exposed animals (p<0.05). Similarly, 28 days of exposure to DDVP led to a significant increase in lactate dehydrogenase, creatinine kinase and troponin (p<0.05). DDVP-exposed rats also showed a significant increase in malondialdehyde, and a significant decline in superoxide dismutase and glutathione peroxidase (p<0.05). However, catalase was comparable in DDVP-exposed and control rats. Histopathological observations of the cardiac tissue revealed that DDVP caused marked fat degeneration and necrosis of the myocardial layer. The changes in DDVP-exposed rats were significantly, though not completely, restored by seed oil administration.
This study provides novel mechanistic information on the cardiotoxicity of DDVP inhalation, and the antioxidant potential of seed oil.
心血管疾病是全球非传染性疾病的主要病因。农药的使用与非传染性疾病的高全球负担有关。尽管人们会通过吸入二氯松（DDVP）而遭受无差别暴露，但目前尚无关于其可能产生心脏毒性的报道。本研究调查了吸入二氯松暴露的心脏毒性以及种子油的可能作用。
21 只雄性大鼠被随机分为 3 组。A 组（对照组）仅接受标准大鼠饮食和自由饮水；B 组（DDVP）除了大鼠饮食和水外，还通过吸入 DDVP 每天 15 分钟；C 组（DDVP+种子油）也接受 B 组的治疗，以及 300mg/kg 的种子油，共 28 天。
DDVP 暴露动物的体重增加和心脏重量明显减少（p<0.05）。同样，28 天的 DDVP 暴露导致乳酸脱氢酶、肌酸激酶和肌钙蛋白显著增加（p<0.05）。DDVP 暴露的大鼠还显示出丙二醛显著增加，超氧化物歧化酶和谷胱甘肽过氧化物酶显著下降（p<0.05）。然而，DDVP 暴露组和对照组的过氧化氢酶相似。心脏组织的组织病理学观察显示，DDVP 导致心肌层明显脂肪变性和坏死。种子油的给药显著但不完全地恢复了 DDVP 暴露大鼠的这些变化。
本研究提供了关于 DDVP 吸入的心脏毒性以及种子油的抗氧化潜力的新的机制信息。
