Saka W A, Oladipo A A, Kolawole O R, Olayioye A, Akhigbe R E
Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Jan;398(1):1003-1012. doi: 10.1007/s00210-024-03333-y. Epub 2024 Aug 3.
Dichlorvos is an organophosphate pesticide that is commonly used for agricultural and domestic control of pests and insects. Despite its usefulness, it exerts reproductive toxicity and induces male sexual dysfunction. On the other hand, curcumin has been reported to improve sexual dysfunction. However, till date, no study has reported the impact of curcumin on dichlorvos-induced sexual dysfunction. This study investigated the effect and associated mechanism of curcumin on dichlorvos-induced sexual dysfunction. Thirty-two male Wistar rats were randomized into four groups; the control (1 mL of olive oil), curcumin-treated (100 mg/kg), DDVP-treated (98.54 g/m of dichlorvos by inhalation), and DDVP + Curcumin-treated. Dichlorvos induced sexual dysfunction as depicted by reduced motivation to mate (8.38 ± 0.18 vs. 4.00 ± 0.33, P < 0.0001), prolonged latencies (46.63 ± 1.30 vs. 98.75 ± 1.32, P < 0.0001) and reduced frequencies of mount (14.88 ± 0.52 vs. 8.63 ± 0.38), intromission (9.38 ± 0.50 vs. 3.75 ± 0.31, P < 0.0001), and ejaculation (7.63 ± 0.38 vs. 1.50 ± 0.19, P < 0.0001). These findings were accompanied by suppression of hypothalamic-pituitary-testicular axis, evidenced by marked reductions in circulating FSH (60.00 ± 1.04 vs. 21.13 ± 0.52, P < 0.0001), LH (46.38 ± 1.38 vs. 19.00 ± 0.46, P < 0.0001), and testosterone (6.01 ± 0.50 vs. 0.74 ± 0.05, P < 0.0001). Nonetheless, the administration of curcumin in dichlorvos-exposed rats significantly attenuated dichlorvos-induced sexual dysfunction by improving the assessed indices of male sexual act. Also, curcumin significantly increased serum levels of FSH (21.13 ± 0.52 vs. 47.25 ± 0.10, P < 0.0001), LH (19.00 ± 0.46 vs. 43.00 ± 1.49), and testosterone (0.74 ± 0.05 vs. 3.98 ± 0.08, P < 0.0001). This study revealed that curcumin attenuated dichlorvos-induced sexual dysfunction by activating the hypothalamic-pituitary-testicular axis and upregulating circulating testosterone.
敌敌畏是一种有机磷农药，常用于农业和家庭害虫防治。尽管其有用，但它具有生殖毒性并会导致男性性功能障碍。另一方面，姜黄素据报道可改善性功能障碍。然而，迄今为止，尚无研究报道姜黄素对敌敌畏诱导的性功能障碍的影响。本研究调查了姜黄素对敌敌畏诱导的性功能障碍的作用及相关机制。32只雄性Wistar大鼠被随机分为四组；对照组（1毫升橄榄油）、姜黄素处理组（100毫克/千克）、敌敌畏处理组（通过吸入98.54微克/立方米敌敌畏）和敌敌畏+姜黄素处理组。敌敌畏诱导了性功能障碍，表现为交配动机降低（8.38±0.18对4.00±0.33，P<0.0001）、潜伏期延长（46.63±1.30对98.75±1.32，P<0.0001）以及爬跨频率降低（14.88±0.52对8.63±0.38）、插入频率降低（9.38±0.50对3.75±0.31，P<0.0001）和射精频率降低（7.63±0.38对1.50±0.19，P<0.0001）。这些结果伴随着下丘脑-垂体-睾丸轴的抑制，表现为循环中促卵泡生成素（FSH）显著降低（60.00±1.04对21.13±0.52，P<0.0001）、促黄体生成素（LH）显著降低（46.38±1.38对19.00±0.46，P<0.0001）和睾酮显著降低（6.01±0.50对0.74±0.05，P<0.0001）。尽管如此，给暴露于敌敌畏的大鼠施用姜黄素通过改善评估的雄性性行为指标，显著减轻了敌敌畏诱导的性功能障碍。此外，姜黄素显著提高了血清FSH水平（21.13±0.52对47.25±0.10，P<0.0001）、LH水平（19.00±0.46对43.00±1.49）和睾酮水平（0.74±0.05对3.98±0.08，P<0.0001）。本研究表明，姜黄素通过激活下丘脑-垂体-睾丸轴和上调循环睾酮来减轻敌敌畏诱导的性功能障碍。
