Traub W H, Spohr M, Bauer D
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Jun;265(1-2):182-96. doi: 10.1016/s0176-6724(87)80165-7.
The minimal intraperitoneal (i.p.) immunogenic doses of phenol-hot water lipopolysaccharide (P-W- LPS) preparations from three strains of Serratia marcescens for juvenile NMRI mice ranged from 3.2 to 16 ng following i.p. challenge infection with homologous strains. Dual autoclaving (121 degrees C, 15 min) abolished the cross-immunogenicity of two selected P-W LPS extracts. Four purified metalloproteases from S. marcescens strains SF 178, SH 186, SV O1, and SE 182 shared the following properties: a) inhibition of proteolytic (azocasein hydrolysis) activity by 50 mM of EDTA; b) heat-lability (60 degrees C, 15 min); c) identical molecular weights (54,000 Daltons = 54 K) as documented with the SDS-PAGE procedure; d) close serologic relatedness (ELISA technique, polyclonal rabbit immune sera); and e) uniform reactivity of the 54 K polypeptide bands with polyclonal rabbit anti-metalloprotease immune sera (Western blots). The minimal immunogenic dose of metalloprotease SF 178, the sole significantly murine immunogenic enzyme, was 1000 ng = 1 microgram.
三种粘质沙雷氏菌菌株的酚-热水平脂多糖（P-W-LPS）制剂对幼年NMRI小鼠的最小腹腔内（i.p.）免疫原性剂量，在经同源菌株腹腔内激发感染后，范围为3.2至16纳克。双重高压灭菌（121摄氏度，15分钟）消除了两种选定的P-W LPS提取物的交叉免疫原性。来自粘质沙雷氏菌菌株SF 178、SH 186、SV O1和SE 182的四种纯化金属蛋白酶具有以下特性：a）50 mM的EDTA抑制蛋白水解（偶氮酪蛋白水解）活性；b）热不稳定性（60摄氏度，15分钟）；c）如SDS-PAGE程序所记录的相同分子量（54,000道尔顿 = 54 K）；d）密切的血清学相关性（ELISA技术，兔多克隆免疫血清）；e）54 K多肽带与兔抗金属蛋白酶多克隆免疫血清（Western印迹）的均匀反应性。唯一具有显著小鼠免疫原性的酶——金属蛋白酶SF 178的最小免疫原性剂量为1000纳克 = 1微克。
