Traub W H, Spohr M, Bauer D
Institut für Medizinische Mikrobiologie und Hygiene, Universität des Saarlandes, Homburg/Saar.
Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Dec;267(2):228-46. doi: 10.1016/s0176-6724(87)80008-1.
The minimal active immunogenic doses (intraperitoneal administration) of crude K-antigen extracts of Serratia marcescens for NMRI mice were 80 ng. Following ultracentrifugation (300,000 x g, 16 h), supernatant fluids of three K-antigen extracts were free of contaminating DNA, RNA, and 2-keto-3-deoxy-D-manno-octonic acid (KDO), and the contents of Limulus amoebocyte lysate-reactive lipopolysaccharide (LPS) had been reduced from 100- to 1000-fold. The minimal active immunogenic doses of two ultracentrifuged K-antigen extracts were 2 and 10 micrograms, respectively. A mucoid strain of S. marcescens (SM 20-M; serotype O6/O14:H12) yielded nonmucoid (= SM 29-NM) variants that had lost most of the O6/O14 O-antigen (LPS) and all of the SM 29-M K-antigen extract reactivity (ELISA test) and which were ca. 5-fold less mouse-virulent. Crude K-antigen extracts from S. marcescens strain SM 29-M and variant SM 29-NM failed to protect NMRI mice against strain SM 29-M.
粘质沙雷氏菌粗K抗原提取物对NMRI小鼠的最小活性免疫原剂量（腹腔注射）为80纳克。经过超速离心（300,000×g，16小时）后，三种K抗原提取物的上清液不含污染的DNA、RNA和2-酮-3-脱氧-D-甘露糖辛酸（KDO），鲎试剂反应性脂多糖（LPS）的含量从100倍降至1000倍。两种超速离心的K抗原提取物的最小活性免疫原剂量分别为2微克和10微克。粘质沙雷氏菌的一个黏液菌株（SM 20-M；血清型O6/O14:H12）产生了非黏液（=SM 29-NM）变体，这些变体失去了大部分O6/O14 O抗原（LPS）以及所有的SM 29-M K抗原提取物反应性（ELISA试验），并且其对小鼠的毒力约低5倍。来自粘质沙雷氏菌菌株SM 29-M和变体SM 29-NM的粗K抗原提取物未能保护NMRI小鼠免受菌株SM 29-M的侵害。
