Luzi Francesca, Tortorella Ilaria, Di Michele Alessandro, Dominici Franco, Argentati Chiara, Morena Francesco, Torre Luigi, Puglia Debora, Martino Sabata
Department of Civil and Environmental Engineering, Materials Engineering Center, UdR INSTM, University of Perugia, Strada di Pentima 4, 05100 Terni, Italy.
Department of Chemistry, Biology and Biotechnologies, University of Perugia, 06123 Perugia, Italy.
Nanomaterials (Basel). 2020 Oct 31;10(11):2176. doi: 10.3390/nano10112176.
Herein we present the production of novel nanocomposite films consisting of polylactic acid (PLA) polymer and the inclusion of nanoparticles of lignin (LNP), ZnO and hybrid ZnO@LNP (ZnO, 3.5% wt, ICP), characterized by similar regular shapes and different diameter distribution (30-70 nm and 100-150 nm, respectively). The obtained set of binary, ternary and quaternary systems were similar in surface wettability and morphology but different in the tensile performance: while the presence of LNP and ZnO in PLA caused a reduction of elastic modulus, stress and deformation at break, the inclusion of ZnO@LNP increased the stiffness and tensile strength (σ = 65.9 MPa and E = 3030 MPa) with respect to neat PLA (σ = 37.4 MPa and E = 2280 MPa). Neat and nanocomposite PLA-derived films were suitable for adult human bone marrow-mesenchymal stem cells and adipose stem cell cultures, as showed by their viability and behavior comparable to control conditions. Both stem cell types adhered to the films' surface by vinculin focal adhesion spots and responded to the films' mechanical properties by orchestrating the F-actin-filamin A interaction. Collectively, our results support the biomedical application of neat- and nanocomposite-PLA films and, based on the absence of toxicity in seeded stem cells, provide a proof of principle of their safety for food packaging purposes.
在此，我们展示了由聚乳酸（PLA）聚合物以及木质素纳米颗粒（LNP）、氧化锌（ZnO）和杂化氧化锌@木质素纳米颗粒（ZnO，3.5%重量，电感耦合等离子体质谱法）组成的新型纳米复合薄膜的制备，其特征在于具有相似的规则形状和不同的直径分布（分别为30 - 70纳米和100 - 150纳米）。所获得的二元、三元和四元体系在表面润湿性和形态方面相似，但在拉伸性能方面有所不同：虽然PLA中LNP和ZnO的存在导致弹性模量、应力和断裂伸长率降低，但与纯PLA（σ = 37.4兆帕和E = 2280兆帕）相比，ZnO@LNP的加入提高了刚度和拉伸强度（σ = 65.9兆帕和E = 3030兆帕）。纯PLA和纳米复合PLA衍生薄膜适用于成人骨髓间充质干细胞和脂肪干细胞培养，其活力和行为与对照条件相当即可证明。两种干细胞类型均通过纽蛋白粘着斑附着于薄膜表面，并通过协调F - 肌动蛋白 - 细丝蛋白A相互作用对薄膜的机械性能做出反应。总体而言，我们的结果支持纯PLA和纳米复合PLA薄膜的生物医学应用，并且基于接种干细胞时无毒性，为其用于食品包装目的的安全性提供了原理证明。
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