Department of Pathology, Chonnam National University Research Institute of Medical Science, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Republic of Korea.
Department of Neurosurgery, Chonnam National University Research Institute of Medical Science, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Republic of Korea.
In Vivo. 2023 Sep-Oct;37(5):2039-2043. doi: 10.21873/invivo.13301.
BACKGROUND/AIM: Urinary bladder cancer has various etiologies and tends to recur and then progress to a higher grade. When muscles are invaded, the response to conventional therapy is poor and the quality of life deteriorates rapidly. Here, we summarize and compare two representative methods used to create the syngeneic mouse models required for immunological research.
In this study, we utilized six-week-old female C3H/HeNCrl mice and the mouse bladder tumor cell line MBT-2. The first method involved transurethral catheterization with poly-L-lysine pretreatment (catheter group), while the second method involved transperitoneal incision and direct injection of tumor cells into the bladder wall (open group). Mouse postoperative status was monitored on a weekly basis using magnetic resonance imaging (MRI).
The catheter group had a tumor development rate of 47% (7 out of 15 mice), with only 1 mouse developing an intravesical tumor. In contrast, the open group had a higher tumor formation rate of 69% (47 out of 68 mice), with 27 mice showing intravesical tumor formation. Notably, with a lower cell count, urinary obstruction events were observed 2 weeks post-inoculation, which is one week later than the higher cell count group.
In this study, we conducted a comparative analysis between the transurethral catheterization method and the transperitoneal incision and direct injection method in animal bladder tumor models. Our findings provide evidence of the consistent effectiveness in constructing a stable model within the open group. Well-designed orthotopic animal models are essential.
背景/目的：膀胱癌有多种病因，容易复发并进展为更高分级。当肌肉受到侵犯时，常规治疗的反应较差，生活质量迅速恶化。在这里，我们总结并比较了两种用于创建免疫研究所需同基因小鼠模型的代表性方法。
在这项研究中，我们使用了 6 周龄雌性 C3H/HeNCrl 小鼠和小鼠膀胱肿瘤细胞系 MBT-2。第一种方法是经尿道膀胱镜检查并用聚赖氨酸预处理（导管组），第二种方法是经腹腔切开并直接将肿瘤细胞注入膀胱壁（开放组）。每周使用磁共振成像（MRI）监测小鼠术后状态。
导管组的肿瘤发展率为 47%（15 只小鼠中有 7 只），只有 1 只小鼠发生膀胱内肿瘤。相比之下，开放组的肿瘤形成率更高，为 69%（68 只小鼠中有 47 只），其中 27 只小鼠出现膀胱内肿瘤形成。值得注意的是，接种后 2 周观察到细胞计数较低的小鼠发生尿路梗阻事件，比细胞计数较高的组晚一周。
在这项研究中，我们比较了经尿道膀胱镜检查法和经腹腔切开直接注射法在动物膀胱肿瘤模型中的应用。我们的发现为开放组中构建稳定模型的一致有效性提供了证据。精心设计的原位动物模型至关重要。
