miRNA-671-5p 通过靶向 NFIA/CRYAB 轴促进前列腺癌的发展和转移。

MiRNA-671-5p Promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis.

机构信息

Department of Urology & Andrology, Minimally Invasive Surgery Center, Guangdong Provincial Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University. Guangzhou, Guangdong, China.

出版信息

Cell Death Dis. 2020 Nov 3;11(11):949. doi: 10.1038/s41419-020-03138-w.

Prostate cancer (PCa) is the second cause of death due to malignancy among men, and metastasis is the leading cause of mortality in patients with PCa. MicroRNAs (miRNAs) play important regulatory roles in tumor development and metastasis. Here, we identified 13 miRNAs related to PCa metastasis by bioinformatics analysis. Moreover, we found that miR-671-5p was increased in metastatic PCa tissues, and its high expression indicated poor prognosis of PCa. MiR-671-5p could facilitate PCa cells proliferation, migration, and invasion in vitro and vivo. We confirmed that miR-671-5p directly bound to the 3' untranslated regions of NFIA mRNA, and NFIA directly bound to the CRYAB promoter. High expression of NFIA and CRYAB negatively correlated with the advanced clinicopathological characteristics and metastasis status of PCa patients. Our study demonstrated that miR-671-5p promoted PCa development and metastasis by suppressing NFIA/ CRYAB axis.

前列腺癌（PCa）是男性恶性肿瘤死亡的第二大原因，而转移是 PCa 患者死亡的主要原因。微小 RNA（miRNA）在肿瘤发生和转移中发挥重要的调节作用。在这里，我们通过生物信息学分析鉴定了 13 个与 PCa 转移相关的 miRNA。此外，我们发现 miR-671-5p 在转移性 PCa 组织中增加，其高表达表明 PCa 的预后不良。miR-671-5p 可以促进 PCa 细胞在体外和体内的增殖、迁移和侵袭。我们证实 miR-671-5p 可以直接结合 NFIA mRNA 的 3'非翻译区，而 NFIA 可以直接结合 CRYAB 启动子。NFIA 和 CRYAB 的高表达与 PCa 患者的晚期临床病理特征和转移状态呈负相关。我们的研究表明，miR-671-5p 通过抑制 NFIA/CRYAB 轴促进 PCa 的发展和转移。