11个圈养猕猴种群的流行病学调查及基因型分析
Epidemiological investigation and genotypes of in 11 captive Rhesus macaque populations.
作者信息
Yu Mengshi, Liu Xue, Xie Meng, Li Diyan, Ni Qingyong, Zhang Mingwang, Wu Jiayun, Xu Huailiang, Yao Yongfang
机构信息
College of Life Science, Sichuan Agricultural University, Ya'an, 625014, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
出版信息
Int J Parasitol Parasites Wildl. 2020 Oct 21;13:191-195. doi: 10.1016/j.ijppaw.2020.10.007. eCollection 2020 Dec.
is an obligate intracellular parasite and the most common pathogen of microsporidiosis in humans and animals. In this study, a total of 198 fecal samples were collected from 11 captive populations of Rhesus macaque in Chinese zoos, to investigate the prevalence and analyze the zoonotic potential of by genotype of the Internal Transcribed Spacer (ITS) gene on the rRNA of via nested PCR. Results showed that the average infection rate of in the 11 populations was 13.6%, and the highest infection rate was 56.5% in the population of Xinjiang Tianshan Zoo. Seven genotypes were identified including 2 known genotypes (D and CM1) and 5 novel genotypes (Mul1, Mul2, Mul3, Mul4 and Mul5). Phylogenetic analysis revealed that the novel genotypes Mul2, Mul3, Mul4 and Mul5 belonged to Group 1 showed the zoonotic potential．These findings extend the distribution of genotypes and provide baseline data for controlling infection.
是一种专性细胞内寄生虫，也是人类和动物微孢子虫病最常见的病原体。在本研究中，从中国动物园的11个恒河猴圈养种群中总共采集了198份粪便样本，通过巢式PCR检测核糖体RNA内部转录间隔区（ITS）基因的基因型，以调查的感染率并分析其人畜共患病潜力。结果显示，11个种群中的平均感染率为13.6%，新疆天山动物园种群的感染率最高，为56.5%。鉴定出7种基因型，包括2种已知基因型（D和CM1）和5种新基因型（Mul1、Mul2、Mul3、Mul4和Mul5）。系统发育分析表明，属于第1组的新基因型Mul2、Mul3、Mul4和Mul5具有人畜共患病潜力。这些发现扩展了基因型的分布范围，并为控制感染提供了基线数据。
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