School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
Biofouling. 2020 Sep;36(8):990-999. doi: 10.1080/08927014.2020.1832219. Epub 2020 Nov 4.
In the study, sp. K6 and were found to form an enhanced biofilm when cultured in the presence of organic acids and sugars. Here, the highest biofilm could be observed for sp. K6 (3.08 ± 0.13) and (1.99 ± 0.12) when cultured in presence of 10 µM malic acid. However, maximum production of indole 3 acetic acid (IAA) was observed with 25 µM succinic acid treatment for sp. K6 (24.33 ± 0.57 µg ml) and with 25 µM galactose for (20 ± 0.0 µg ml). At the same time, sp. K6 solubilized the highest quantity of phosphate in the presence of 50 µM citric acid (21.33 ± 0.0 µM) and was observed to produce 32.66 ± 1.25 µM soluble phosphate in the presence of 10 µM galactose. The results of the study demonstrate the role of organic acids and sugars in the enhancement of biofilm formation, IAA production and phosphate solubilization in selected spp. and highlight the potential use of rhizobacteria in conjugation with supplement for the agricultural applications.
在这项研究中，当在有机酸和糖存在的情况下培养时，sp. K6 和 被发现形成增强的生物膜。在这里，当在 10 μM 苹果酸存在的情况下培养时，可以观察到最高的生物膜形成，sp. K6 为 3.08 ± 0.13， 为 1.99 ± 0.12。然而，当用 25 μM 琥珀酸处理 sp. K6 时，观察到吲哚 3 乙酸（IAA）的最大产量为 24.33 ± 0.57 μg ml，而用 25 μM 半乳糖处理 时，产量为 20 ± 0.0 μg ml。同时，在 50 μM 柠檬酸存在的情况下，sp. K6 溶解了最多的磷酸盐（21.33 ± 0.0 μM），而 在 10 μM 半乳糖存在的情况下，观察到 产生了 32.66 ± 1.25 μM 可溶性磷酸盐。研究结果表明，有机酸和糖在增强选定 spp. 的生物膜形成、IAA 产生和磷酸盐溶解中的作用，并强调了根际细菌与补充剂在农业应用中的潜在用途。
