Parappilly Sherin Joy, Radhakrishnan E K, George Sumi Mary
Post Graduate and Research Department of Microbiology, Sree Sankara College, Kalady, 683574, Kerala, India.
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
Braz J Microbiol. 2024 Dec;55(4):3529-3539. doi: 10.1007/s42770-024-01530-8. Epub 2024 Oct 4.
The present study focused on the antibacterial and antibiofilm activity of novel lactic acid bacterial (LAB) strains isolated from the healthy human volunteers of different age groups and their consortium (LABCON), against the enteropathogenic bacteria. From the study, methanolic extract of LAB isolates and their consortia were found to have promising antibacterial activity and antibiofilm activity against Escherichia coli (ATCC 35218) and Staphylococcus aureus (ATCC 25923). The antimicrobial compounds including the DL-3 phenyllactic acid, DL-p-hydroxyphenyllactic acid, and Succinic acid produced by the LAB could be considered to inhibit the growth and biofilm formation by E. coli (ATCC 35218) and S. aureus (ATCC 25923). Detailed insight into the antibiofilm activity could also be demonstrated by Confocal Raman microscopy attached with AFM and Fluorescent microscope. From the results of the study, the consortium LABCON was superior in antimicrobial and antibiofilm activity and can be considered to have promising application in infection control.
本研究聚焦于从不同年龄组健康人类志愿者中分离出的新型乳酸菌（LAB）菌株及其联合体（LABCON）对肠道致病菌的抗菌和抗生物膜活性。通过该研究发现，LAB分离株及其联合体的甲醇提取物对大肠杆菌（ATCC 35218）和金黄色葡萄球菌（ATCC 25923）具有可观的抗菌活性和抗生物膜活性。LAB产生的包括DL-3-苯乳酸、DL-p-羟基苯乳酸和琥珀酸在内的抗菌化合物可被认为能抑制大肠杆菌（ATCC 35218）和金黄色葡萄球菌（ATCC 25923）的生长及生物膜形成。结合原子力显微镜（AFM）和荧光显微镜的共聚焦拉曼显微镜也能详细揭示抗生物膜活性。从研究结果来看，联合体LABCON在抗菌和抗生物膜活性方面表现更优，可被认为在感染控制方面具有广阔的应用前景。
