Gansu Gaolan Field Scientific Observation and Research Station for Agricultural Ecosystem, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China.
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2022 Feb 1;23(3):1694. doi: 10.3390/ijms23031694.
L. is an important traditional Chinese medicinal herb now in demand worldwide. Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown. Here, we applied three concentrations of both EZ99 inoculant (1.0 × 10, 1.0 × 10, and 1.0 × 10 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on in a field experiment. The results showed that LB + MS (1.0 × 10 CFU/mL + 1.5 g/L sucrose) and LB + LS (1.0 × 10 CFU/mL + 0.15 g/L sucrose) treatments significantly increased root fresh weight ( ≤ 0.05). Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%. Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil. Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen spp. showed an effective suppression. Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of associated with change in potassium bioavailability. Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of , and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential. Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
党参是一种重要的传统中药，目前在全球范围内需求旺盛。最近的理论框架表明，蔗糖会触发植物根际促生菌（PGPM）的定植，但它们在植物上的相互作用在很大程度上仍不清楚。在这里，我们应用了三种浓度的 EZ99 接种剂（1.0×10、1.0×10 和 1.0×10 菌落形成单位（CFU）/mL，分别表示为 LB、MB 和 HB）和蔗糖（0.15、1.5 和 15 g/L，分别表示为 LS、MS 和 HS），在田间试验中研究它们对 的共效应。结果表明，LB+MS（1.0×10 CFU/mL+1.5 g/L 蔗糖）和 LB+LS（1.0×10 CFU/mL+0.15 g/L 蔗糖）处理显著增加了根鲜重（ ≤ 0.05）。代谢物分析显示，LB+LS 处理显著增加了大黄中主要活性成分的相对含量，即蒽醌类和酚类化合物，分别增加了 1.5%和 2.3%。虽然高蔗糖添加增加了某些土壤酶的活性，但 LB+LS 处理显著增加了总钾（TK），而降低了有效钾（AK），这有利于根际土壤中钾的利用。此外，根际微生物组学表明，LB+LS 处理中真菌多样性增加，其中常见的致病真菌 spp. 得到了有效抑制。此外，冗余分析和 Spearman 相关性表明，与钾生物有效性变化相关的 与 呈正相关。总之，我们的研究结果表明，细菌接种剂和蔗糖的联合应用可以促进党参的生长和品质，刺激植物养分的吸收，从而改变微生物群落，发挥生物防治潜力。因此，这项工作不仅在经济作物中有广泛的应用前景，而且强调了农业生态实践在可持续农业中的重要性。
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